结球甘蓝BoFBX117基因的克隆与表达分析

F-box基因在植物组织器官发育及非生物胁迫响应中起着重要的作用.为进一步探讨其作用机制,从结球甘蓝品种BoJF-16-1中利用反转录PCR(RT-PCR)技术克隆得到一个F-box基因BoFBX117,其cDNA全长为936 bp,编码311个氨基酸,蛋白分子量为30.14 kDa,等电点为9.71.该基因编码BoF...

Cloning and Expression Analysis of BoFBX117 From Brassica oleracea

F-box gene plays an important role in plant tissue development and abiotic stress response. To further explore the mechanism, a F-box gene BoFBX117 was cloned from the cabbage variety "BoJF-16-1" by RT-PCR. Its cDNA was 936 bp in length, encoding 311 amino acids, protein molecular weight was 30.14 kDa, and isoelectric point was 9.71. The gene encodes BoFBP7 protein. Sequence homology analysis showed that BoFBP7 is closely related to other FBP7 proteins in turnip, radish and Arabidopsis, with homology of 99.36%, 98.39%, and 92.26%, respectively. Quantitative PCR analysis showed that the expression of BoFBX117 gene was the highest in the rosette leaves, followed by the roots, and the lowest was in the young leaves, suggesting the tissue specific expression of BoFBX117 gene in cabbage. Under low temperature stress, the expression of BoFBX117 gene showed an upward trend, indicating that the expression of this gene was induced by low temperature stress. It is suggested that the BoFBX117 gene may play an important role in leaf development and response to low temperature stress in cabbage. This will provide basis for further analysis of the molecular mechanism of F-box gene regulating plant development and abiotic stress.