| 快速溶剂萃取技术在2种食药用真菌多糖提取中的应用 |
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| 引用本文： | 闫佳佳,万璐,吴桐,郑春英. 快速溶剂萃取技术在2种食药用真菌多糖提取中的应用[J]. 中国农学通报, 2021, 37(16): 150-155. DOI: 10.11924/j.issn.1000-6850.casb2020-0429 |
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| 作者姓名： | 闫佳佳 万璐 吴桐 郑春英 |
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| 作者单位： | 1.黑龙江大学农业微生物技术教育部工程研究中心,哈尔滨 150500;2.黑龙江大学生命科学学院/黑龙江省普通高校微生物重点实验室,哈尔滨 150080;3.黑龙江大学生命科学学院/黑龙江省普通高校分子生物学重点实验室,哈尔滨 150080 |
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| 基金项目： | 黑龙江大学校企合作横向项目“一株产原儿茶酸的枯草芽孢杆菌”(20170223) |
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| 摘 要： | 为建立快速溶剂萃取技术提取食药用真菌多糖的新方法,以猪苓、茯苓为研究对象,采用单因素及正交实验优选快速溶剂萃取法提取多糖的最佳工艺条件.结果 表明,快速溶剂萃取法用于猪苓多糖提取的最佳工艺参数为提取温度110℃,提取压力110 bar,提取时间20 min,循环2次,在此条件下,猪苓多糖的含量为(7.8684士0.10...
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| 关 键 词： | 快速溶剂萃取法 猪苓多糖 茯苓多糖 提取工艺 单因素实验 正交实验 |
| 收稿时间： | 2020-09-07 |
Application of Pressurized Solvent Extraction Technology in the Extraction of Polysaccharide from Two Kinds of Edible and Medicinal Fungi |
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| Yan Jiajia,Wan Lu,Wu Tong,Zheng Chunying. Application of Pressurized Solvent Extraction Technology in the Extraction of Polysaccharide from Two Kinds of Edible and Medicinal Fungi[J]. Chinese Agricultural Science Bulletin, 2021, 37(16): 150-155. DOI: 10.11924/j.issn.1000-6850.casb2020-0429 |
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| Authors: | Yan Jiajia Wan Lu Wu Tong Zheng Chunying |
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| Affiliation: | 1.Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500;2.Key Laboratory of Microbiology, College of Heilongjiang Province/ School of Life Sciences, Heilongjiang University, Harbin 150080;3.Key Laboratory of Molecular Biology, College of Heilongjiang Province/ School of Life Sciences, Heilongjiang University, Harbin 150080 |
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| Abstract: | The paper aims to develop Pressurized Solvent Extraction (PSE) for extracting polysaccharides in two kinds of edible and medicinal fungi with the Polyporus umbellatus and Poria cocos were chosen as raw materials, the single factor and orthogonal experiments were used to explore the optimal conditions for the extraction of polysaccharide by PSE. The results showed that the optimized parameters of PSE employed temperature 110℃, pressure 110 bar, and extracting time was set at 20 min for 2 times to extract polysaccharides in Polyporus umbellatus completely. Under the condition, the content of Polyporus umbellatus polysaccharide was (7.8684±0.1096) mg/g. Meanwhile, the optimized parameters of PSE employed temperature 110℃, pressure 120 bar, and extracting time was set at 25 min for 2 times to extract Poria cocos polysaccharides completely. Under the condition, the content of Poria cocos polysaccharide was (8.6965±0.1087) mg/g. As a conclusion, PSE can be used to extract polysaccharides from the two kinds of edible and medicinal fungi quickly and accurately. |
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| Keywords: | PSE Polyporus umbellatus polysaccharides Poria cocos polysaccharides extraction technology single factor experiment orthogonal experiment |
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