晋麦47 EMS突变体库的构建及高代突变材料品质性状的初步分析

为加快小麦抗旱种质资源创制,推进抗旱品种的遗传改良工作,采用0.6％甲基磺酸乙酯(EMS)溶液处理晋麦47的种子,构建了包含12272个单株的M2突变体库,对各突变性状和突变率进行调查与分析.此外,对前期研究筛选出的50份M7和M8突变材料籽粒的硬度指数、蛋白质含量、湿面筋含量、沉降值、吸水率、稳定时间、最大拉伸阻力和...

Construction of Ethyl Methane Sulfonate Mutant Library of Jinmai 47 and Preliminary Analysis of Quality Characteristics of High Generation Mutants

To speed up creating of drought-resistant wheat resources and promote the genetic improvement of drought-resistant wheat varieties, the seeds of Jinmai 47 were treated with 0.6% EMS solution, a M₂ mutant library containing 12272 individual plants was constructed, and the mutation characters and mutation rates were investigated. In addition, the grain hardness index, protein content, wet gluten content, sedimentation value, water absorption, stability time, maximum resistance and extensive area of 50 M₇ and M₈ mutants selected in the previous study were analyzed. The results showed that: M₂ mutant library was rich in phenotypic variation, including stem character, fertility, spike type, disease resistance, leaf character, growth period and awn character, and the total mutation rate was 30.24%. Among which, the mutation rates of fertility, plant height and panicle shape were higher. There were not only morphological mutations in grain shape, grain length, plumpness and grain color, but also variations in grain quality characters of 50 mutants. Among them, the coefficient of variation of stability time, extensive area, maximum resistance and sedimentation value was the highest, the coefficient of variation of hardness index, protein content and wet gluten content was medium, and the coefficient of variation of water absorption was the lowest. Except for water absorption, positive variation occurred in a certain proportion of other characters. Four mutant materials with good quality characters were preliminarily screened, except hardness index and water γ-absorption, the other six quality characters were better than those of the wild type. This research provides valuable genetic materials for the discovery of wheat functional genes and new ideas for the drought resistance breeding program.