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四川茂县花椒根腐病病原菌的分离与鉴定
引用本文:李志克,唐蓉,黄小琴,张蕾,张重梅,鲜赟曦,何天伟,周西全,刘勇,杨潇湘. 四川茂县花椒根腐病病原菌的分离与鉴定[J]. 中国农学通报, 2021, 37(27): 100-104. DOI: 10.11924/j.issn.1000-6850.casb2021-0308
作者姓名:李志克  唐蓉  黄小琴  张蕾  张重梅  鲜赟曦  何天伟  周西全  刘勇  杨潇湘
作者单位:1.四川省农业科学院植物保护研究所,农业农村部西南作物有害生物综合治理重点实验室,成都 610066;2.茂县林业和草原局,四川茂县 623200;3.营山县农业农村局,四川营山 637700
基金项目:四川省农业科学院青年领军人才研究基金“茂县花椒根腐病的病原鉴定及其生物学特性研究”(2020LJRC007)
摘    要:旨在明确引起阿坝藏族羌族自治州茂县花椒根腐病的病原菌种类,为科学有效防治该病提供理论参考.以采自茂县的花椒根腐病病根为材料,通过组织分离法、致病性测定、形态学观察与基于核糖体转录间隔区序列(ITS)和延长因子基因(tef1)的分子鉴定相结合进行病原菌的分离鉴定.结果 表明,病原菌菌丝初为灰白色,后为兰褐色或紫红色.大型...

关 键 词:花椒  根腐病  病原真菌  分离鉴定  腐皮镰孢菌
收稿时间:2021-03-24

The Pathogen Causing Root Rot in Zanthoxylum bungeanum in Mao County of Sichuan Province: Isolation and Identification
Li Zhike,Tang Rong,Huang Xiaoqin,Zhang Lei,Zhang Zhongmei,Xian Yunxi,He Tianwei,Zhou Xiquan,Liu Yong,Yang Xiaoxiang. The Pathogen Causing Root Rot in Zanthoxylum bungeanum in Mao County of Sichuan Province: Isolation and Identification[J]. Chinese Agricultural Science Bulletin, 2021, 37(27): 100-104. DOI: 10.11924/j.issn.1000-6850.casb2021-0308
Authors:Li Zhike  Tang Rong  Huang Xiaoqin  Zhang Lei  Zhang Zhongmei  Xian Yunxi  He Tianwei  Zhou Xiquan  Liu Yong  Yang Xiaoxiang
Affiliation:1.Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Key Laboratory of Integrated Pest Management in Southwest Agriculture Crops of Ministry of Agriculture and Rural Affairs, Chengdu 610066;2.Forestry and Grassland Bureau of Mao County, Mao County Sichuan 623200;3.Agriculture and Rural Bureau of Yingshan County, Yingshan Sichuan 637700
Abstract:To identify the pathogen species causing root rot in Zanthoxylum bungeanum in Mao County, Aba Tibetan and Qiang Autonomous Prefecture, and provide theoretical reference for scientific and effective treatment of this disease, the rot roots of Z. bungeanum were collected from Mao County and the pathogen was isolated and identified by tissue isolation method, pathogenicity determination, morphological characteristics and molecular identification which was based on ITS and tef1 gene sequences. The results showed that the mycelium of pathogen was gray white at the beginning, and then became blue brown or purple red. Large conidia of the pathogen were sickle-shaped and small conidia were kidney-shaped. Phylogenetic analysis based on ITS and tef1 gene sequences showed that the pathogen was attributed to the same branch with Fusarium solani in the phylogenetic tree. According to morphology and molecular identification, the pathogen of root rot in Z. bungeanum in Mao County of Sichuan Province was identified as F. solani.
Keywords:Zanthoxylum bungeanum  root rot  pathogenic fungi  isolation and identification  Fusarium solani  
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