欧李种质叶片类黄酮含量及不同组分特征研究

为筛选出欧李叶片类黄酮和9种类黄酮物质中单一物质含量较高的品种,并分析确定具有抗氧化和抑制酪氨酸酶活性的物质,本研究以38份欧李种质基生枝成熟期叶片为试材,利用超高效液相色谱法(UHPLC)测定儿茶素、表儿茶素、甘草素、芦丁、槲皮素、槲皮素-7-O-葡萄糖苷、杨梅素、光甘草定、根皮素活性物质含量,对其抗氧化及抑制酪氨酸...

Analysis of Flavonoids Content and Different Components in Leaves of Chinese Dwarf Cherry Germplasm

To screen out the varieties with the highest flavonoid content and substance with antioxidant and tyrosinase activity in leaves of Chinese Dwarf Cherry. This study used mature leaves from 38 Chinese Dwarf Cherry germplasms as materials, the concentrations of catechin, epicatechin, liquiritigenin, rutin, quercetin, quercetin -7-O- glucoside, myricetin, glabridin and phloretin were determined by Ultra Performance Liquid Chromatography (UHPLC), and the antioxidant activity and the tyrosinase inhibitory ability were analyzed. The results showed that the flavonoid content of variety Y09-14 was 64.84 mg·g^-1, the highest among 38 germplasms. Moreover, the Coefficient of variation of flavonoid and its 9 components among all varieties were over 20%, indicating there were rich genetic diversities. Cluster analysis based on flavonoid content in leaves showed that more than 70% of germplasms were classed as medium flavonoid level. By measuring the content of flavonoids and different components in leaves of different germplasms, it was found that catechin, epicatechin, rutin and quercetin -7-O- glucoside could be detected in all 38 germplasms, and catechin content was 6.271-935.295 mg·100g^-1, which was significantly higher than the content of other eight substances. Catechin, epicatechin, rutin and quercetin -7-O- glucoside were positively correlated with DPPH scavenging rate, indicating that these four components had strong antioxidant capacity. Rutin, quercetin -7-O- glucoside and glabridin were positively correlated with tyrosinase inhibition rate. This study provides the basis for the detection and extraction of related substances in Chinese Dwarf Cherry in future studies.