液氮冻结软壳青蟹在冻藏期间的营养物质变化

为探讨液氮(LN)冻结拟穴青蟹软壳蟹在冻藏期间的营养物质变化,本研究采用基于核磁共振(NMR)的代谢组学技术分析了LN冻结软壳蟹可食组织代谢谱在短期(7 d)和长期(8个月)冻藏期间的变化.结果表明,-20℃冻藏和LN冻结均对短期冻藏的软壳蟹代谢谱无显著影响(P＞0.05),但-20℃冻藏导致软壳蟹肌肉的二甲胺和肝胰腺...

Effect of Liquid Nitrogen Freezing on Changes in Nutrients of Soft-Shell Crabs of Scylla paramamosain During Its Frozen Storage

To better understand the effect of liquid nitrogen (LN) freezing on changes in nutrients of soft-shell green mud crab (Scylla paramamosain) with during its frozen storage, a nuclear magnetic resonance (NMR)-based metabolomics technique was used to monitor metabolic profiles of edible tissues of soft-shell crabs with LN freezing during short-term (7 days) and long-term (8 months) frozen storage(-20℃). The results showed that short-term frozen storage and LN freezing showed no significant effect on crab metabolic profiles. However, the muscle dimethylamine level was tripled whereas the hepatopancreas inosine level was doubled after short-term frozen storage. In contrast, LN freezing could inhibit the increase of these two metabolites. Besides, long-term frozen storage with LN freezing lead to a significant increase in the muscle inosine, maltose, and glucose as well as hepatopancreas inosine, glutamine, and uridine of soft-shell crabs. These findings demonstrated the effectiveness of LN freezing to ameliorate the quality deterioration of soft-shell crab during its frozen storage. This study provide a theoretical reference for understanding the quality change of soft shell crobs during frozen storage.