山东省生姜细菌性黄叶斑病病原鉴定

Identification of the pathogen causing ginger bacterial yellow leaf spot disease in Shandong

Ginger (Zingiber officinale Roscoe) is an important economic crop in China. Bacterial leaf spot disease is the major limiting factor for the ginger crop production in Shandong. In recent years, a bacterial leaf spot disease was observed on ginger in Laiwu and Anqiu, that became a major limiting factor in ginger production. Yellowish bacterial colonies that were obtained after incubation at 28°C for 2 days from infected leaves were purified by streaking on nutrient agar. The purified isolates were initially identified as Pantoea ananatis by the phenotypic and biochemical characterization. Further molecular identification based on the 16S rDNA sequence analysis and multilocus sequence analysis with the gyrB, rpoB, and atpD sequences confirmed that the isolates were P. ananatis. To our knowledge, this is the first report of bacterial leaf spot of ginger caused by P. ananatis in Shandong. Our study will provide basic references for disease management of this pathogen.