湛江等鞭金藻藻际细菌群落与其耐高温关系分析

为解析湛江等鞭金藻(Isochrysis zhangjiangensis)野生藻株(IZ)与常压室温等离子体技术诱变获得的耐高温诱变藻株(IM)藻际细菌群落与耐高温能力的关联性,本研究采用16S rDNA扩增子测序方法对两株藻在25℃和35℃下细菌群落多样性进行分析.ACE和Chao1指数分析表明,25℃下培养两株藻的...

Analysis of Relationship Between Bacterial Community and High Temperature Tolerance of Isochrysis zhangjiangensis

To analyze a correlation between high-temperature resistance and the bacterial community associated with Isochrysis zhangjiangensis, the bacterial community of wild microalgae strain I. zhangjiangensis(IZ)and the high-temperature resistant mutant strain(IM)induced by atmospheric and room temperature plasmas at 25℃ and 35℃ by 16S rDNA amplification sequencing was analysed. No significant difference in the composition of the bacterial community was found between two strains at 25℃ as indicated by the ACE and Chao1 index, while the bacterial community diversity of IM group(IM35L)was significantly higher than that of IZ group at 35℃(IZ35L). NMDS analysis showed that the bacterial community of IM group(IM25L)and IZ group at 25℃(IZ25L)was similar, which was significantly different at 35℃. In addition, the top three genera of bacteria in the IM25L, IZ25L and IZ35L groups was the same, but the bacteria affiliated with the genera Algoriphagus was only dominant in the IM35L group. Moreover, MetaStat indicated that the abundance of Alpha proteobacterium DG1252, a bacterium of Proteobacterium Rhodospirillaceae, illuminated no significant difference between IZ and IM group at 25℃, while it was greater than in the IM group at 35℃. Therefore, a correlation of the high temperature tolerance and its bacterial diversity of IM was existed. the bacteria affiliated with the genera Algoriphagus and Alpha proteobacterium DG1252 might be closely related to the high temperature resistance of IM. The study aided in exploring the state of knowledge for the relationship between the high temperature stress resistance and bacterial community associated with I. Zhangjiangensis, which was helpful for the cultivation and propagation of shellfish microalgae under high temperature conditions in summer.