超高压提取黄精多糖及提高运动耐力机制

为确定炮制黄精多糖(PPP)的最佳提取工艺,本试验以黄精为原料,进行炮制,利用超高压技术提取PPP,分析了压力强度、保压时间、料液比对PPP提取率的影响,采用Box-Behnken响应面分析法(RSM)优化PPP提取条件,并对所得多糖进行动物体内试验,分析小鼠血清、肝脏和左腿腓肠肌中相关酶的活性变化,探究其提高机体运动耐力机制。结果表明,PPP最佳提取工艺条件为:液料比23 mL·g^-1、保压时间6.73 min,压力强度293 MPa,在此条件下PPP得率为13.46%;能显著提高小鼠力竭游泳时间(P<0.05),相比于游泳对照组,小鼠血清中尿素氮(SUN)、乳酸(Lac)和肝脏丙二醛(MDA)含量显著下降(P<0.05),同时乳酸脱氢酶(LDH)活性下降、Ca²⁺Mg²⁺-ATP酶及肌酸激酶(CK)活性上升,对提高小鼠运动耐力具有较好的效果。本研究结果为超高压提取PPP奠定了基础,并为PPP提高运动耐力的机制研究提供了数据支持。

Ultrahigh Pressure Extraction of Polygonatum sibiricum Polysaccharide and Mechanism of Improving Exercise Endurance

In order to determine the optimal extraction process of polygonatum polysaccharide (PPP), Polygonatum was used as the raw material using ultra-high pressure technology after processed. The effect of pressure strength, pressure holding time, solid-liquid ratio on PPP extraction rate were analyzed. Box-Behnken response surface analysis (RSM) was used to optimize the PPP extraction conditions, and the obtained polysaccharides were tested in vivo to analyze the changes in the content of related enzymes in serum, liver and left leg skeletal muscle in mice. The mechanism of improving exercise endurance was investigated. The results showed that the optimal extraction process conditions for PPP were: liquid-material ratio 23 pressure holding time 6.73 min, pressure intensity 293 MPa. Under these conditions, the PPP yield was 13.46%; which could significantly improve the exhausted swimming time of mice (P<0.05). Compared with the swimming control group, the contents of SUN, Lac and MDA in mice were significantly decreased (P<0.05), At the same time lactate dehydrogenase (LDH) decreased, ATPase and CK increased, which had a better effect of improving exercise endurance, and provided data support for the mechanism of PPP to improve exercise endurance.