中华蜜蜂精液的冷冻贮存技术(英文)

中华蜜蜂精液在液氮（－ １９６ ℃） 中贮存１２１ ｄ，精液与含有８％ 二甲亚砜的三羟基氨基甲烷缓冲稀释液以１∶１．５ 混合，不用平衡，在广口保温瓶中距液氮面２ ｃｍ 或５ ｃｍ 处预冻，在３５～３９ ℃水浴中解冻，可产生较好的解冻后活力比率（超过６０％ ）．当室温高于２０ ℃时（４ 月份），样本必须于１３ ℃下平衡０．５ ｈ 左右，其解冻后活力比率可达３０％ ．春季采集并冷冻的样本，其解冻后活力不如冬季采集并冷冻的．用在液氮中贮存了１～１２１ ｄ 的精液分别以 ４～５ μ Ｌ的剂量给 ２４ 只处女王人工授精，４ 只开产但首先产的不是受精卵．解冻后的精液能够于１３ ℃下保存４６ ｈ．磺胺不能用于精子冷冻．

Freezing preservation technique of honeybee semen

Honeybee (Apis cerana cerana) semen was stored for 121 days at -196 ℃ in liquid nitrogen.Samples consistng of semen and tris buffer diluent containing 8% dimethyl sulphoxide,at 2 or 5 cm over the liquid nitrogen surface in a broadmouth thermos for 5-10 min to prefreeze and at 35-39 ℃ water bath to thaw,could produce over 60% post-thaw motility rate without equilibration(the radio between semen and tris buffer diluent was 1∶1.5).When the room temperature went beyond 20 ℃ (in April),samples must be equilibrated at 13 ℃ for 0.5 h or so,the post-thaw motility rate of the freezing sample would be over 30%.The post-thaw motility of samples collected and frozen in spring were not so good as that in winter,twenty four virgin queens were inseminated instrumentally with 4-5 μL semen stored for 1-121 days in liquid nitrogen,four of these queens laid but didn,t lay fertilized eggs first.Frozen semen could be stored for 46 h at 13 ℃ after thawing.Sulphanilamide should not be used in semen freezing.