| 根癌农杆菌介导的香石竹遗传转化体系建立 |
| |
| 引用本文： | 孔维龙,王翠,但乃震,李丹丹,包满珠,傅小鹏.根癌农杆菌介导的香石竹遗传转化体系建立[J].中国农业大学学报,2018,23(4):34-44. |
| |
| 作者姓名： | 孔维龙 王翠 但乃震 李丹丹 包满珠 傅小鹏 |
| |
| 作者单位： | 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室/农业部华中都市农业重点实验室;北京百迈客生物科技有限公司;石河子大学农学院; |
| |
| 基金项目： | 国家自然科学基金项目（31000918）；中央高校基本科研业务费专项（2662015PY052，2662016PY041） |
| |
| 摘 要： | 为获得香石竹最佳转化体系及稳定表达的转基因植株,以香石竹组培苗叶片为材料,研究农杆菌菌种、香石竹基因型、预培养天数、侵染方式、共培养方式和时间等多个因素对香石竹遗传转化的影响。结果表明:香石竹转化的最佳方式是预培养2d,采用LBA4404农杆菌缓冲液摇床摇12min(200r/min),静置3min的侵染方式。侵染后叶片外植体接种于50μmol/L乙酰丁香酮(AS)溶液浸湿的无菌滤纸(3层),进行黑暗共培养5d。不同基因型对比结果表明:‘小桃红'的抗性芽分化率优于‘马斯特'和‘锦葵钛合金'。60mg/L卡那霉素(Kan)浓度对3个基因型的香石竹品种均有较好的抗性芽筛选效果,0.5mg/L吲哚丁酸(IBA)+0.22mg/L噻苯隆(TDZ)培养基是分化效率高、丛芽多、生长状态好的直接出芽培养基。本研究建立的的香石竹遗传转化体系,重复性好、转化效率高、基因型依赖较小,该体系能显著提高DcaPIP1;1基因的转化效率。
|
| 关 键 词： | 香石竹 农杆菌介导 遗传转化体系 高效 |
| 收稿时间： | 2017/6/8 0:00:00 |
Agrobacterium-mediated genetic transformation system of carnation |
| |
| KONG Weilong,WANG Cui,DAN Naizhen,LI Dandan,BAO Manzhu and FU Xiaopeng.Agrobacterium-mediated genetic transformation system of carnation[J].Journal of China Agricultural University,2018,23(4):34-44. |
| |
| Authors: | KONG Weilong WANG Cui DAN Naizhen LI Dandan BAO Manzhu and FU Xiaopeng |
| |
| Institution: | College of Horticulture and Forestry Sciences/Key Laboratory of Horticultural Plant Biology of Ministry of Education/Key Laboratory of Urban Agricuture in Central China of Ministroy of Agriaulture, Huazhong Agriculture University, Wuhan 430070, China;Biomarker Technologies, Beijing 101300, China,College of Agronomy, Shihezi University, Shihezi 832003, China,College of Horticulture and Forestry Sciences/Key Laboratory of Horticultural Plant Biology of Ministry of Education/Key Laboratory of Urban Agricuture in Central China of Ministroy of Agriaulture, Huazhong Agriculture University, Wuhan 430070, China,College of Horticulture and Forestry Sciences/Key Laboratory of Horticultural Plant Biology of Ministry of Education/Key Laboratory of Urban Agricuture in Central China of Ministroy of Agriaulture, Huazhong Agriculture University, Wuhan 430070, China,College of Horticulture and Forestry Sciences/Key Laboratory of Horticultural Plant Biology of Ministry of Education/Key Laboratory of Urban Agricuture in Central China of Ministroy of Agriaulture, Huazhong Agriculture University, Wuhan 430070, China and College of Horticulture and Forestry Sciences/Key Laboratory of Horticultural Plant Biology of Ministry of Education/Key Laboratory of Urban Agricuture in Central China of Ministroy of Agriaulture, Huazhong Agriculture University, Wuhan 430070, China |
| |
| Abstract: | |
| |
| Keywords: | carnation agrobacterium-mediated genetic transformation system high efficiency |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国农业大学学报》浏览原始摘要信息 |
| 点击此处可从《中国农业大学学报》下载免费的PDF全文 |
|
