| 拟南芥翻译起始因子eIF5B-1调控种子萌发的分子机制 |
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| 引用本文： | 寇小霞,张丽媛,赵月,田雪军,郭伟龙,辛明明,彭惠茹.拟南芥翻译起始因子eIF5B-1调控种子萌发的分子机制[J].中国农业大学学报,2018,23(1):12-18. |
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| 作者姓名： | 寇小霞 张丽媛 赵月 田雪军 郭伟龙 辛明明 彭惠茹 |
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| 作者单位： | 中国农业大学农学与生物技术学院/农业生物技术国家重点实验室/杂种优势研究与利用教育部重点实验室; |
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| 基金项目： | 国家自然科学基金项目（31571747）；转基因重大专项（2016ZX08002-002） |
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| 摘 要： | 为明确eIF5B-1基因在拟南芥种子萌发过程中的生理作用。以拟南芥翻译起始因子的T-DNA插入突变体eif5b-1为材料。测定突变体eif5b-1种子的萌发速率,同时利用多聚核糖体谱和qRT-PCR的研究方法,对拟南芥野生型(WT)和eif5b-1突变体的萌发过程进行了研究。结果表明:1)eif5b-1突变体种子的萌发率下降;2)eif5b-1突变体种子萌发过程中多聚核糖体的形成受到影响;3)eif5b-1突变体中ABI1、ABI3、ABI4、ABI5及DOG1基因的转录受到影响;4)eif5b-1突变体选择性地影响ABI4和DOG1基因的翻译效率。
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| 关 键 词： | 拟南芥 翻译起始因子 种子 萌发 翻译 脱落酸 |
| 收稿时间： | 2016/11/9 0:00:00 |
Molecular regulatory mechanism of Arabidopsis translation initiation factor eIF5B-1 on seeds germination |
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| KOU Xiaoxi,ZHANG Liyuan,ZHAO Yue,TIAN Xuejun,GUO Weilong,XIN Mingming and PENG Huiru.Molecular regulatory mechanism of Arabidopsis translation initiation factor eIF5B-1 on seeds germination[J].Journal of China Agricultural University,2018,23(1):12-18. |
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| Authors: | KOU Xiaoxi ZHANG Liyuan ZHAO Yue TIAN Xuejun GUO Weilong XIN Mingming and PENG Huiru |
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| Institution: | College of Agronomy and Biotechnology/State Key Laboratory forAgrobiotechnology/Key Laboratory of Crop Heterosis and Utilization(Ministry of Education), China Agricultural University, Beijing 100193, China,College of Agronomy and Biotechnology/State Key Laboratory forAgrobiotechnology/Key Laboratory of Crop Heterosis and Utilization(Ministry of Education), China Agricultural University, Beijing 100193, China,College of Agronomy and Biotechnology/State Key Laboratory forAgrobiotechnology/Key Laboratory of Crop Heterosis and Utilization(Ministry of Education), China Agricultural University, Beijing 100193, China,College of Agronomy and Biotechnology/State Key Laboratory forAgrobiotechnology/Key Laboratory of Crop Heterosis and Utilization(Ministry of Education), China Agricultural University, Beijing 100193, China,College of Agronomy and Biotechnology/State Key Laboratory forAgrobiotechnology/Key Laboratory of Crop Heterosis and Utilization(Ministry of Education), China Agricultural University, Beijing 100193, China,College of Agronomy and Biotechnology/State Key Laboratory forAgrobiotechnology/Key Laboratory of Crop Heterosis and Utilization(Ministry of Education), China Agricultural University, Beijing 100193, China and College of Agronomy and Biotechnology/State Key Laboratory forAgrobiotechnology/Key Laboratory of Crop Heterosis and Utilization(Ministry of Education), China Agricultural University, Beijing 100193, China |
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| Abstract: | |
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| Keywords: | Arabidopsis translation initiation factor seeds germination translation ABA |
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