| 粉枝莓ISSR遗传多样性和果实香气成分多样性分析 |
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| 引用本文： | 刘瑜,钟政昌,袁雷,谢博,曹东星.粉枝莓ISSR遗传多样性和果实香气成分多样性分析[J].中国农业大学学报,2018,23(8):50-58. |
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| 作者姓名： | 刘瑜 钟政昌 袁雷 谢博 曹东星 |
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| 作者单位： | 西藏农牧学院食品科学学院;西藏森林资源天然产物研究中心;西藏农牧学院生物技术中心 |
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| 基金项目： | 西藏自治区自然科学基金项目（2015ZR-13-30）；西藏野生特色生物资源开发平台建设项目（PT2015-01） |
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| 摘 要： | 为明确西藏粉枝莓的多样性和亲缘关系,利用ISSR分子标记技术和HS-SPME/GC-MS技术,对粉枝莓7个居群的遗传多样性和果实香气成分多样性进行研究。结果表明:11条引物共得到102个清晰的扩增条带,其中多态性条带99个,PPB达到97.06%,香农信息指数I为0.490 9,表明粉枝莓具有较高的遗传多样性水平。Nei’s遗传结构(GST,0.287 2)和分子变异分析(AMOVA)的结果表明大部分的遗传变异分布在居群内(71.28%)。通过遗传分化系数GST估计的基因流为1.241 1。45个粉枝莓果实共检测出28种挥发性成分,基于果实香气成分的欧式距离为0.028 6~0.144 6。利用Mantel检验结果表明粉枝莓ISSR遗传距离和果实香气成分欧式距离无显著相关性(r=-0.153 2,P=0.676 0)。采用28种香气成分对45个样本进行主成分分析,前4个主成分的累积贡献率达到74.20%,较好的反映了粉枝莓果实香气成分的组成情况。研究表明,粉枝莓存在较丰富的遗传多样性,为西藏粉枝莓资源的进一步研究和利用提供参考。
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| 关 键 词： | 粉枝莓 ISSR标记 香气成分 主成分分析 |
| 收稿时间： | 2017/10/19 0:00:00 |
Genetic and phytochemical diversities of Rubus biflorus Buch revealed by ISSR markers and fruit aroma components |
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| LIU Yu,ZHONG Zhengchang,YUAN Lei,XIE Bo and CAO Dongxing.Genetic and phytochemical diversities of Rubus biflorus Buch revealed by ISSR markers and fruit aroma components[J].Journal of China Agricultural University,2018,23(8):50-58. |
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| Authors: | LIU Yu ZHONG Zhengchang YUAN Lei XIE Bo and CAO Dongxing |
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| Institution: | Food Science College, Tibet Agricultural & Animal Husbandry University, Linzhi 860000, China,Food Science College, Tibet Agricultural & Animal Husbandry University, Linzhi 860000, China;Tibet Natural Products Research Center of Forest Resources, Linzhi 860000, China,Tibet Natural Products Research Center of Forest Resources, Linzhi 860000, China;Biotechnology Center, Tibet Agricultural & Animal Husbandry University, Linzhi 860000, China,Food Science College, Tibet Agricultural & Animal Husbandry University, Linzhi 860000, China and Food Science College, Tibet Agricultural & Animal Husbandry University, Linzhi 860000, China |
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| Abstract: | To clarify the diversity and relationship of Rubus biflorus Buch,the genetic and phytochemical diversities of 7 populations of Rubus biflorus Buch were investigated using ISSR and HS-SPME/GC-MS.The results showed that:Among102 clear amplified bands obtained by 11 pairs primers,99 were polymorphic.The number of polymorphic bands accounted for 96.07% of total bands,the Shannon index of diversity I was 0.490 9,indicating that Rubus biflorus Buch had abundant genetic diversity.The coefficient of gene differentiation (GST,0.365 2) and AMOVA analysis revealed that most genetic diversities were distributed within populations (71.28%).The estimate of gene flow based on GST was 1.241 1.28 aromatic compounds were detected among 45 Rubus biflorus Buch in total.The Euclidean distance among the seven populations varied from 0.028 6-0.144 6.The Mantel test showed that there was no significant correlation between the genetic distance and Euclidean distance (r=-0.153 2,P=0.676 0).Principal component analysis was used to evaluate the 28 aromatic components.The results indicated that the former 4 principal components accounted for 74.20% of the cumulative contribution value.In conclusion,PCA could simplify the complicate original data to principal components,which reflected the aroma constitution preferably.This study showed that there existed a rich genetic diversity providing references for further research and utilization of Rubus biflorus Buch resources. |
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| Keywords: | Rubus biflorus Buch ISSR marker aroma components principal component analysis |
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