Molecular cloning of a Mycoplasma meleagridis-specific antigenic domain endowed with a serodiagnostic potential |
| |
Authors: | Mardassi B Ben Abdelmoumen Béjaoui Khiari A Oussaief L Landoulsi A Brik C Mlik B Amouna F |
| |
Institution: | Laboratoire des Mycoplasmes, Institut Pasteur de Tunis, 13 Place Pasteur, B.P. 74, 1002 Tunis-Belvédère, Tunis, Tunisia. Boutheina.mardassi@pasteur.rns.tn |
| |
Abstract: | A recombinant phage library harbouring Mycoplasma meleagridis (MM) genomic DNA fragments was generated in the bacteriophage lambda gt11 expression vector. The library was screened for expression of MM specific antigens with a polyclonal antiserum that had been preadsorbed with antigens of the most common unrelated avian mycoplasma species. A 49-amino acid antigenic domain unique to MM was isolated, expressed in Escherichia coli, and its serodiagnostic potential was demonstrated. An antiserum raised against this MM-specific antigenic domain recognized a cluster of seven membrane-associated MM proteins with molecular masses ranging from 34 to 75 kDa. Overall, this study resulted in the identification of a potent serodiagnostic tool and revealed the complex antigenic nature of MM. |
| |
Keywords: | Mycoplasma meleagridis Genomic library Species-specific epitopes Fusion protein Serodiagnosis |
本文献已被 ScienceDirect PubMed 等数据库收录! |