膜荚黄芪不定根组织培养的研究

[目的]建立黄芪不定根培养的无性繁殖体系。[方法]以膜荚黄芪无菌苗的胚根为外植体,诱导了不定根。将长约1 cm的不定根切段接种于4种培养基(B5、MS、LS和White)以筛选培养基。然后以B5为基本培养基附加不同浓度IBA(1~6 mg/L)、不同种类糖(蔗糖、葡萄糖、果糖和食用白糖)和不同浓度白糖(20~50 g),研究IBA浓度和糖的种类及浓度对黄芪不定根组织培养的影响。[结果]4种培养基中,B5培养基效果最好,切段可分化大量不定根,且生长良好。IBA浓度为2 mg/L时,黄芪不定根生长旺盛。在B5+IBA 2.0 mg/L培养基上,附加30 g/L蔗糖或白糖时不定根生长良好,白糖与蔗糖的效果差异不明显。[结论]在黄芪不定根规模化生产中可用白糖代替培养基中蔗糖,降低培养基成本。

Preliminary Study on the Tissue Culture of Adventitious Root in Astragalus membrcmaceus

[Objective] The study aimed to establish the clone propagation system of adventitious root culture in Astragalus membrcmaceus.[Method] With radicels of aseptic seedlings of A.membrcmaceus as explants,the adventitious roots were induced.The segments with length of about 1 cm of adventitious roots were inoculated on 4 media(B5,MS,LS and White) to screen media.Then IBA with different concn.(1～6 mg/L),different kinds of sugar(sucrose, glucose,fructose and edible white sugar) and white sugar with different concn.(20～50 g) were added into B5 basic media to study the influences of IBA concn.,the kind and concn.of sugar on the tissue culture of adventitious roots in A.membrcmaceus.[Result] Among 4 kinds of media,the effect of B5 medium was best,on which,the segments could differentiate a lot of adventitious roots and they grew well.When IBA was 2 mg/L,the A.membrcmaceus adventitious root grew prosperously.On the medium of B5+IBA 2.0 mg/L,when 30 g/L sucrose or white sugar was added,the adventitious root grew well.The difference on the effects of white sugar and sucrose was not significant.[Conclusion] In the scale production of A.membrcmaceus adventitious roots,the sucrose in media could be replaced by white sugar to reduce medium cost.