紫外诱变选育普鲁兰多糖高产菌株 |
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引用本文: | 李慧颖,张璐,刘丽芳,孔维涛,程书梅.紫外诱变选育普鲁兰多糖高产菌株[J].安徽农业科学,2011,39(29):17795-17797. |
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作者姓名: | 李慧颖 张璐 刘丽芳 孔维涛 程书梅 |
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作者单位: | 河北农业大学食品科学学院,河北保定,071000 |
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摘 要: | 目的]选育普鲁兰多糖的高产菌株。方法]以Aureobasidium pullulan NNG为出发菌株,采用紫外诱变的方法,筛选普鲁兰多糖高产菌株。结果]出发菌株A.pullulan NNG摇瓶发酵所得普鲁兰多糖产量为28.79 g/L,而突变菌株UV-1产糖量为46.96 g/L,是出发菌株的1.69倍。通过突变株UV-1与出发菌株NNG发酵期内形态的观察,发现在发酵的第7天突变株UV-1膨胀细胞数达到最高。结论]该研究可以为普鲁兰多糖高产菌株的筛选提供理论依据。
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关 键 词: | 出芽短梗霉 普鲁兰多糖 紫外诱变 |
Study on the Selection of Pullulan High-yield Strains by Ultraviolet Mutation |
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Institution: | LI Hui-ying et al(College of Food Science and Technology,Agricultural University of Hebei,Baoding,Hebei 071000) |
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Abstract: | Objective]The research aimed to study the selection of pullulan high-yield strains.Method]Taken Aureobasidium pullulan NNG as original strain,pullulan high-yield strains were selected by using ultraviolet mutation.Result]The results showed that the yield of pullulan of mutant strain UV-1 under shaking condition was 46.96 g/L,it was 1.69 times of that of A.pullulan NNG,which was 28.79 g/L.And the observation results on the shapes of original strains and mutation strains showed that mutation strain UV-1 had the highest quantity of expansive cells on the 7th day of fermentation.Conclusion]This research could provide scientific basis for the selection of pullulan high-yield strains. |
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Keywords: | Aureobasidium pullulan Pullulan Ultraviolet mutation |
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