粘毛黄芩丛生芽的诱导及快速繁殖 |
| |
引用本文: | 王淑芳,李凤华,窦全丽.粘毛黄芩丛生芽的诱导及快速繁殖[J].安徽农业科学,2011,39(23):14022+14097-14022,14097. |
| |
作者姓名: | 王淑芳 李凤华 窦全丽 |
| |
作者单位: | 遵义师范学院生物系,贵州遵义,563002;遵义师范学院生物系,贵州遵义,563002;遵义师范学院生物系,贵州遵义,563002 |
| |
基金项目: | 遵义市科技局资助项目“根癌农杆菌介导的CryIA抗虫基因转化生姜的研究”(【2010】16号) |
| |
摘 要: | 目的]为粘毛黄芩种质保存和工厂化繁育提供理论依据。方法]以粘毛黄芩无菌苗的幼茎为外植体,MS为基本培养基,分别对其进行启动、丛生芽及生根培养,建立粘毛黄芩离体培养再生体系。结果]粘毛黄芩最佳丛生芽诱导培养基MS+6-BA 2.0 mg/L+IAA 0.2 mg/L,丛生芽分化率可达94.8%,平均丛生芽倍增数为11.6;试管苗生根培养基以1/2 MS+IAA 0.5 mg/L最好,生根率高达97.8%。结论]该研究建立了粘毛黄芩的快速、高效无性繁殖体系。
|
关 键 词: | 粘毛黄芩 丛生芽诱导 快速繁殖 |
Induction and Rapid Propagation of Scutellauria viscidula Shoots |
| |
Institution: | WANG Shu-fang et al(Biology Department,Zunyi Normal College,Zunyi,Guizhou 563002) |
| |
Abstract: | Objective]To provide theoretical basis for the germplasm cryopreservation and factory propagation of Scutellaria viscidula Bunge..Method]With the tender stems of Scutellaria viscidula Bunge as explants and MS as basic medium for their starting,shoots and rooting culture,the regeneration system for its in vitro culture was constructed.Result]The optimal induction medium for Scutellaria viscidula shoots was 2.0 mg/L 6-BA and 0.2 mg/L IAA,the average differentiated rate of shoots was 94.8% with the average reproduction multiples of 11.6;the best rooting culture medium was 1/2MS + IAA 0.5 mg/L,with the highest rooting rate of 97.8%.Conclusion]The study established a rapid and high-efficient asexual regeneration system for Scutellaria viscidula Bunge. |
| |
Keywords: | Scutellaria viscidula Bunge Shoots induction Rapid propagation |
本文献已被 CNKI 维普 万方数据 等数据库收录! |
|