| 肉牛肌生成抑制素成熟蛋白编码序列的克隆与原核表达 |
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| 引用本文： | 左明坤,沈冰蕾,倪洪波.肉牛肌生成抑制素成熟蛋白编码序列的克隆与原核表达[J].安徽农业科学,2011,39(29):17920-17922. |
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| 作者姓名： | 左明坤 沈冰蕾 倪洪波 |
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| 作者单位： | 黑龙江八一农垦大学动物科技学院,黑龙江大庆,163319 |
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| 摘 要： | 目的]原核表达肉牛肌生成抑制素成熟蛋白编码序列基因并进行功能验证,为后期的小鼠接种实验打下基础。方法]采用RT-PCR方法扩增肉牛MSTN成熟蛋白编码序列基因,该扩增产物经过双酶切后克隆到表达载体pET28a（＋）中,转化大肠杆菌BL21,经IPTG诱导表达后采用SDS-PAGE和Western-blot检测重组目的蛋白的表达结果。结果]经Anti-His-Tag鉴定正确。结论]肉牛MSTN成熟蛋白编码序列基因在原核表达系统中得到正确表达。
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| 关 键 词： | 肉牛 肌生成抑制素 克隆 原核表达 生物活性 |
Cloning and Prokaryotic Expression of the Coding Sequence of Maturation Protein of Bovine Myostatin Gene |
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| Institution: | ZUO Ming-kun et al(College of Animal Science and Technology,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319) |
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| Abstract: | Objective] The aim was to test and verify maturation protein of bovine myostatin(MSTN) gene based on prokaryotic expression,to provide basis for inoculation experiment of mice.Method] RT-PCR method was used to amplify coding sequence of maturation protein of bovine myostatin(MSTN) gene,then after double digestion,amplification products were cloned to expression vector pET28a(+),transform escherichia coli BL21,after induction of IPTG,SDS-PAGE and Western-blot were used to detect expression of results of recombinant protein.Result] Anti-His-Tag identification was correct.Conclusion] Beef cattle MSTN were expressed in prokaryotic expression system correctly. |
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| Keywords: | Beef cattle Mysotatin Cloning Prokaryotic expression Bioactivity |
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