青海湖地区4种荒漠植物抗逆性指标的研究

[目的]研究青海湖地区4种荒漠植物的抗逆性。[方法]通过比较中麻黄、狼毒、芨芨草、黄缨菊4种植物的抗逆性指标（可溶性蛋白、MDA、游离Pro含量以及SOD、POD、CAT活性）,分析青海湖地区4种植物的抗逆性,并运用Fuzzy隶属函数法对4种植物抗逆性进行综合评价。[结果]青海湖地区低温、干旱逆境下,4种荒漠植物的SOD活性表现为狼毒〉黄缨菊〉中麻黄〉芨芨草;CAT活性表现为狼毒〉黄缨菊〉芨芨草〉中麻黄,相同的高盐度环境下,狼毒的CAT活性比中麻黄要高出8倍,说明狼毒的抗盐性要强于中麻黄;游离Pro含量表现为黄缨菊〉中麻黄〉狼毒〉芨芨草,相同逆境下中麻黄的Pro含量是狼毒的2.83倍,这是由于狼毒的MDA含量最低,体内膜质过氧化伤害最小,导致胁迫渗透物质Pro含量低,也说明狼毒的抗逆性强于中麻黄。Fuzzy隶属函数综合评价表明,4种荒漠植物的抗逆性表现为黄缨菊〉狼毒〉中麻黄〉芨芨草。[结论]该研究为青海湖地区抗旱新品种的选育和优良种质资源的开发提供了理论依据。

Study on the Resistance Indexes of Four Kinds of Desert Plants in Qinghai Lake Area

[Objective] The research aimed to study the resistance of four kinds of desert plants in Qinhai Lake area.[Method] By contrasting the resistance indexes of four kinds of plants which included Ephedra intermedia,Stellera chamaejasme,Achnatherum splendens and Xanthopappus subacaulis,the resistance of four kinds of plants in Qinghai Lake area was analyzed.The resistance indexes included the soluble protein,MDA,free Pro content and the activities of SOD,POD,CAT.Moreover,the resistance of four kinds of plants was evaluated comprehensively by using Fuzzy membership function method.[Result] Under the low temperature and drought environment in Qinghai Lake area,SOD activity presented as Stellera chamaejasme>Xanthopappus subacaulis>Ephedra intermedia>Achnatherum splendens,and CAT activity presented as Stellera chamaejasme>Xanthopappus subacaulis>Achnatherum splendens>Ephedra intermedia.Under the same high-salt environment,CAT activity of Stellera chamaejasme was eight times higher than that of Ephedra intermedia.It illustrated that the salt resistance of Stellera chamaejasme was stronger than that of Ephedra intermedia.The free Pro content showed as Xanthopappus subacaulis>Ephedra intermedia>Stellera chamaejasme>Achnatherum splendens.Under the same adverse environment,Pro content of Ephedra intermedia was 2.83 times of that of Stellera chamaejasme.It was because that MDA content in Stellera chamaejasme was the lowest,and the membranous overoxidation harm was the smallest.It caused that Pro content(stress osmotica material) was low.It also illustrated that the resistance of Stellera chamaejasme was stronger than that of Ephedra intermedia.The comprehensive evaluation of Fuzzy membership function showed that the resistance presented as Xanthopappus subacaulis>Stellera chamaejasme>Ephedra intermedia>Achnatherum splendens.[Conclusion] The research provided the theory basis for the breeding of drought-resistance new variety and the development of good germ plasm resource in Qinghai Lake area.