| 海南木莲叶和枝总异黄酮的含量测定 |
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| 引用本文： | 鲍长余,范超君,陈湛娟,毕和平.海南木莲叶和枝总异黄酮的含量测定[J].安徽农业科学,2011,39(25):15270-15271,15274. |
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| 作者姓名： | 鲍长余 范超君 陈湛娟 毕和平 |
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| 作者单位： | 海南师范大学化学与化工学院;海南海口571158;海南师范大学化学与化工学院;海南海口571158;海南师范大学“省部共建-热带药用植物化学”教育部重点实验室,海南海口571158 |
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| 基金项目： | 海南省教育厅资助项目，海南师范大学大学生创新实验项目 |
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| 摘 要： | 目的]测定海南木莲叶和枝中总异黄酮的含量。方法]以染料木素为对照品,采用紫外分光光度法在266 nm处测定样品的吸光度。结果]染料木素对照品在20~100μg时呈良好线性关系。其回归方程为C=0.003 9A,R=0.999 9。海南木莲叶、枝中总异黄酮的含量为0.240%和0.078%。结论]该方法操作简便,结果稳定,重复性好,适合海南木莲叶和枝中总异黄酮含量的测定。
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| 关 键 词： | 紫外分光光度法 海南木莲 总异黄酮 |
Determination of Isoflavones in the Leaves and Stems of Manglietia hainanensis |
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| Institution: | BAO Chang-yu et al(Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education,Hainan Normal University,Haikou,Hainan 571158) |
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| Abstract: | Objective] The aim was to determine the content of the isoflavones in the leaves and the stems of Manglietia hainanensis.Methods] Genistein was viewed as reference substance,the ultraviolet spectrophotometric method was used to determine the content of the isoflavones in the leaves and branches of Manglietia hainanensis.Results]The calibration curve was linear in the range of 0.000 5-0.004 5 μg/ml,and the regression equation was C=0.003 9A,R=0.999 9,the content was 0.240 % and 0.078 %.Conclusion] The spectrophotometry was suitable for the determination of isoflavones from the leaves and branches of Manglietia hainanensis.This method operated conveniently,the result was stable and has good repeatability. |
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| Keywords: | Ultraviolet spectrophotometric method Manglietia hainanensis Isoflavones |
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