菊花茎尖克隆体外繁殖研究

目的]研究不同因子对菊花芽苗体外繁殖的影响。方法]以秋菊品种＂绣球＂和＂妃子笑＂的顶芽或半木质化茎段为外植体,研究不同激素及组合对菊花芽苗体外繁殖的影响。结果]在MS＋BA 0.5 mg/L＋IBA 0.1 mg/L的诱导培养基上两品种均能诱导分化出芽。在芽的生长增殖阶段,接种到MS＋BA 1.5 mg/L＋IBA 0.5 mg/L＋GA 0.1 mg/L的增殖培养基上,＂红绣球＂和＂妃子笑＂的增殖系数分别可达6.8和6.4,芽苗生长速度快,生长健壮。在1/2 MS＋IBA 0.5 mg/L的生根培养基上,生根所用时间短,根系数量大且粗细始终,移栽成活率较高。结论]该研究为＂绣球＂和＂妃子笑＂菊花品种的组培快繁提供了理论与技术支持。

Study on the Micropropagation of Shoots of Dendranthema morifolium

Objective]The research aimed to study the influences of different factors on the in vitro propagation of shoots of Dendranthema morifolium.Method]By using the apical buds and cutting stems with buds of two chrysanthemum species of "Xiuqiu" and "Feizixiao" as explants,the effects of different hormones recombination at different concentration on the clone propagation in vitro were studied.Result]The multiplication rate of adventitious buds from explants of "Xiuqiu" and "Feizixiao" can reach 6.8 and 6.4 respectively on the mediums supplemented with BA 1.5 mg/L,IBA 0.5 mg/L and GA 0.1 mg/L.Furthermore,the condition of chrysanthemum shoots root induction had been studied.The results indicated that the best condition of rooting is 1/2MS basal medium supplemented with 0.5 mg/L IBA.Conclusion]This research could provide scientific and technological basis for the tissue culture and rapid propagation of "Xiuqiu" and "Fei zixiao".