重金属胁迫培养对微生物蛋白质含量的影响

[目的]研究重金属胁迫培养对微生物蛋白质含量的影响。[方法]选择4种典型微生物（大肠杆菌、枯草芽孢杆菌、啤酒酵母、链霉菌）为试验材料,采用传统微生物培养方法,在不同浓度的Hg2＋、Cd2＋、Cr6＋和Pb2＋重金属离子下进行胁迫培养,通过测定4种典型微生物的蛋白质含量,探讨重金属胁迫对4种微生物蛋白质合成的影响。[结果]低浓度的重金属对4种微生物蛋白质的合成有一定的促进作用,随着重金属浓度的增加,微生物蛋白质的合成均受到不同程度的抑制。枯草芽孢杆菌对这4种重金属的耐受性比其他3种微生物要强,4种重金属离子在比较大的质量浓度范围内（5～50 mg/L）对枯草芽孢杆菌蛋白质的合成有促进作用。低浓度Cr6＋对大肠杆菌蛋白质的合成有较强的促进作用;Pb2＋对大肠杆菌蛋白质合成有明显的抑制作用,对其他3种微生物蛋白质合成在一定浓度范围内有促进作用;低浓度的Cd2＋对4种微生物蛋白质的合成都有促进作用。[结论]该研究为探讨微生物对重金属胁迫的生理响应提供了理论依据。

Effects of Heavy Metal Stress on the Protein Content of Microorganisms

[Objective] The aim was to study the effects of heavy metal stress on the protein content of microorganisms.[Method] By using traditional microbiological culture method,four typical microorganisms(including Escherichia coli,Bacillus subtilis,Saccharomyces cerevisiae Hansen and Streptomycetaceae) were cultured under the stress of heavy metal ions(like Hg2+,Cd2+,Cr6+ and Pb2+) with different concentrations,and the effects of heavy metal stress on the synthesis of protein in four typical microorganisms was discussed through measuring protein content.[Result] Heavy metals with low concentration was beneficial to the synthesis of protein in four typical microorganisms to a certain extent,but the synthesis of protein in four typical microorganisms was inhibited differently with the increase of heavy metal concentration.The tolerance of B.subtilis to the four heavy metals was stronger compared with other three microorganisms,and the four heavy metals with concentration of 5-50 mg/L could promote the protein synthesis of B.subtilis.Cr6+ with low concentration could promote the protein synthesis of E.coli greatly;Pb2+ inhibited the protein synthesis of E.coli obviously,and promoted the synthesis of protein in other three microorganisms under certain concentration;Cd2+ with low concentration promoted the protein synthesis of four microorganisms.[Conclusion] The study could provide theoretical foundation for discussing the physiological response of microorganism to heavy metal stress.