锂元素对雏鸡学习记忆的影响

[目的]研究锂元素在雏鸡学习记忆中的作用。[方法]设定不同浓度锂元素处理鸡胚,待鸡胚孵化后,利用经典的行为学模型,研究不同锂元素浓度对雏鸡学习记忆的影响,通过RT-PCR方法检测学习记忆相关基因jun的表达,分析锂元素是否对jun有影响。[结果]不同浓度锂元素对于雏鸡一次性回避的影响明显,在5 min（短时记忆）、120 min（长时记忆）2个时间点均能够记住这个厌恶反应,与空白对照组相比,注射100 mmol/L氯化锂组的雏鸡在5、120 min 2个时间点均显示有显著或极显著差异（P5m in=0.039〈0.05,P120m in=0.002〈0.01）;jun基因的在训练前和训练后以及不同浓度的锂元素梯度下出现了明显的表达差异。[结论]锂元素在雏鸡的学习记忆中发挥中重要的作用;jun基因是学习记忆环路中的一个重要基因。

Effects of Chronic Lithium Treatment on the Learning and Memory of Chicks

[Objective]To study the effect of chronic lithium treatment on the learning and memory in chicks.[Method]The embryos of chicks were treated in different concentration of lithium.After hatched,the classical model of behavior was adopted to study the effects of lithium treatment on the learning and memory of chicks,and then RT-PCR was used to detect the expression of learning and memory-related gene jun and analyze whether jun was affected by lithium or not.[Result]Different concentrations of lithium had obvious influence on the one-trial avoidance reaction of chicks,the aversive response was recorded in both short term memory(5 min) and long term memory(10min);compared with the control group,the chicks injected with 100 mmol lithium chloride showed significant or extremely significant difference at both the fifth minute(P5min=0.039<0.05,P120min=0.002<0.01);meanwhile,the expression of jun also showed a significant difference in different concentrations of lithium before and after training.[Conclusion] Lithium played an important role in the learning and memory of chicks;and jun was an important gene in the regulation of learning and memory circle.