| 桉树葡萄糖-6-磷酸脱氢酶(G6PDH)的全基因组分析与进化研究 |
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| 引用本文： | 林元震,张志毅,林善枝,刘纯鑫. 桉树葡萄糖-6-磷酸脱氢酶(G6PDH)的全基因组分析与进化研究[J]. 安徽农业科学, 2011, 39(29): 17804-17805,17906 |
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| 作者姓名： | 林元震 张志毅 林善枝 刘纯鑫 |
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| 作者单位： | 华南农业大学林学院,广东广州510642;北京林业大学林木花卉遗传育种教育部重点实验室,北京100083;北京林业大学林木花卉遗传育种教育部重点实验室,北京,100083;华南农业大学林学院,广东广州,510642 |
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| 基金项目： | 广东省教育厅育苗工程(LYM10040);北京林业大学林木育种国家工程实验室开放课题(FOP2010-4) |
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| 摘 要： | [目的]对桉树基因组中葡萄糖-6-磷酸脱氢酶（G6PDH）进行全基因组分析与进化研究。[方法]利用巨桉（Eucalyptus grandsis）全基因组数据,采用BLAST等生物信息学软件分析桉树G6PDH的基因特性、蛋白序列、系统进化树以及启动子特征。[结果]桉树基因组内存在6个G6PDH基因,其编码蛋白中,1个为胞质型G6PDH,5个为质体型G6PDH,而且桉树G6PDH均含有保守基序motif 1、motif 2、motif 3、motif 7、motif 9和motif 11。此外,桉树G6PDH启动子序列中均存在TATA框、增强子与涉及光应答、激素应答、胁迫应答等调控元件。[结论]可为下一步研究桉树G6PDH家族基因的分子功能提供参考。
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| 关 键 词： | 按树 葡萄糖-6-磷酸脱氢酶 进化分析 保守基序 |
Genome-wide Analysis of Glucose-6-phosphate Dehydrogenase(G6PDH) and Its Evolution in Eucalyptus grandsis |
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| Affiliation: | LIN Yuan-zhen et al(College of Forestry,South China Agricultural University,Guangzhou,Guangdong 510642) |
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| Abstract: | [Objective] The aim of this study was to perform genome-wide analysis of glucose-6-phosphate dehydrogenase(G6PDH) and reveal its evolution in Eucalyptus grandsis.[Method] The gene character,protein sequence and phylogenetic tree of G6PDH gene were analyzed by BLAST and other bioinformatics software within Eucalyptus grandsis whole genome database.[Result] Six G6PDH genes,including one cytomic type and five plastids,were detected in the E.grandsis genome.All the G6PDHs had conserved motifs of motif 1,motif 2,motif 3,motif 7,motif 9 and motif 11.Furthermore,promoter sequences of all E.grandsis G6PDH contained TATA box,enhancer,light-responsive,hormone-responsive and stress-responsive regulatory elements.[Conclusion] This study provided reference for further revealing molecular function of E.grandsis G6PDH gene family. |
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| Keywords: | Eucalyptus grandsis Glucose-6-phosphate dehydrogenase Evolution analysis Conserved motif |
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