| 1株抑烟草青枯病生防菌的筛选·鉴定及其活性物质研究 |
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| 引用本文： | 董昆明,陈亮,周晓见,靳翠丽,缪莉.1株抑烟草青枯病生防菌的筛选·鉴定及其活性物质研究[J].安徽农业科学,2011,39(31):19172-19175. |
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| 作者姓名： | 董昆明 陈亮 周晓见 靳翠丽 缪莉 |
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| 作者单位： | 1. 扬州大学环境科学与工程学院,江苏扬州225127;扬州大学海洋科学与技术研究所,江苏扬州225127
2. 扬州大学环境科学与工程学院,江苏扬州,225127 |
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| 基金项目： | 国家自然科学基金项目(41076097,41006097);江苏省环境材料与环境工程重点实验室开放基金项目(K090025,K090027) |
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| 摘 要： | 目的]对1株抑烟草青枯病生防菌进行筛选、鉴定和活性物质研究。方法]采用系列稀释法和平板涂布法从黔江烟区土壤中分离得到菌株,并通过薄层层析、硅胶柱层析、HPLC等手段对其活性物质进行研究。结果]分离得到1株编号为5B18的菌株,其对烟草青枯病病原菌（Ralstonia Solancearum）有强烈拮抗作用。全细胞脂肪酸及16SrDNA分析结果表明,该菌为解淀粉芽孢杆菌（Bacillus amyloliquefaciens）。对该菌提取物分析表明,该提取物为13种不同的化合物组成的混合物。结论]该研究为从微生物角度防治烟草青枯病提供理论基础。
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| 关 键 词： | 烟草青枯病 活性物质 解淀粉芽孢杆菌 |
Study on the Selection,Identification and Its Active Substances of A Biocontroi Bacterium against Tobacco Bacterial Wilt |
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| Institution: | DONG Kun-ming et al(School of Environmental Science and Engineering,Yangzhou University,Yangzhou,Jiangsu 225127) |
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| Abstract: | Objective]The paper was to study the selection,identification and its active substances of a biocontrol bacterium against tobacco bacterial wilt.Method]The strain was isolated from the soil of tobacco cultivation region in Qianjiang by using serial dilution method and spread-plate method.The strain's active substances were studied by TLC,silica gel column chromatography and HPLC.Result]A strain marked as 5B18 was isolated and proved to have significant antagonism to Ralstonia Solancearum.The whole cell fatty acid and 16SrDNA analysis indicated the strain was Bacillus amyloliquefaciens.Analysis of the strain extract showed that the extract was a complicated mixture of 13 different compounds.Conclusion]The research could provide theoretical foundation for the control of tobacco bacterial wilt from the perspective of microbiology. |
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| Keywords: | Tobacco bacterial wilt Active substance Bacillus amyloliquefaciens |
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