| 链霉素抗性筛选井冈霉素高产菌株的研究 |
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| 引用本文： | 牛莉娜,葛翠凤,葛绍荣. 链霉素抗性筛选井冈霉素高产菌株的研究[J]. 安徽农业科学, 2007, 35(28): 8930-8932 |
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| 作者姓名： | 牛莉娜 葛翠凤 葛绍荣 |
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| 作者单位： | 四川大学生物资源与生态环境教育部重点实验室,四川成都,610064 |
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| 摘 要： | 对井冈霉素产生菌吸水链霉菌井冈变种SJ-6(Streptomyce shygroscopicusvar. Jingganggensis Yen)进行UV和亚硝酸复合诱变,在含有菌株孢子最小抑制浓度的链霉素培养基平板上,采用链霉素抗性筛选法获得大量的链霉素抗性突变株,筛选到突变株UN-80,在摇瓶发酵条件下,井冈霉素化学效价达15 943μg/ml,比出发菌株SJ-6提高了84.4%。该菌株连续培养5代,其化学效价基本稳定,具有较好的遗传稳定性。
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| 关 键 词： | 链霉素抗性筛选 UV诱变 亚硝酸诱变 井冈霉素 |
| 文章编号： | 0517-6611（2007）28-08930-03 |
| 修稿时间： | 2007-05-09 |
Study on the Streptomycin Resistance Screening of High-yield Strain of Jinggangmycin |
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| NIU Li-na et al. Study on the Streptomycin Resistance Screening of High-yield Strain of Jinggangmycin[J]. Journal of Anhui Agricultural Sciences, 2007, 35(28): 8930-8932 |
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| Authors: | NIU Li-na et al |
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| Affiliation: | Key Laboratory of Bio-resources and Eco-environment;Ministry of Education;Sichuan University;Chengdu;Sichuan 610064 |
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| Abstract: | UV and nitrous acid complex mutagenesis was conducted on Streptomyces hygroscopicus var.Jingganggensis Yen SJ-6 produced Jinggangmycin.In the medium plate with streptomycin at minimum inhibiting concentration of strain spores,a lot of streptomycin resistant mutants were obtained by streptomycin resistance screening,including the mutant UN-80.Under the condition of shaking flask fermentation,the Jinggangmycin chemical titer reached 15 943 μg/ml,increased 84.4% than the original strain SJ-6.After continuously cultured for 5 generations the strain had basically stable chemical titer and better hereditary stability. |
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| Keywords: | Streptomycin resistance screening UV mutagenesis Nitrous acid mutagenesis Jinggangmycin |
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