酸性电解水对低温条件下单增李斯特菌杀灭效果

单核细胞增生李斯特菌是一种嗜冷菌,能在低温下生长。冷藏即食食品和冷冻食品加工设备中单核细胞增生李斯特氏菌的生长是主要的食品安全问题。酸性电解水 ( AEW ) 是一种新型高效,安全且无残留的非热灭菌技术。AEW用于灭活4 ℃条件下的浮游态、生物膜态及冷藏金针菇样品上单核细胞增生李斯特菌。通过检测AEW处理前后菌落总数变化、生物膜、生物膜结构参数,毒力基因表达,进一步分析样品实验等。结果表明,当电解质浓度为0.1 %,处理时间为1 min时,4 ℃和37 ℃下的单核细胞增生李斯特菌菌落总数、生物被膜清除率存在极显著差异。研究结果丰富了我们对AEW灭活低温条件下的单核细胞增生李斯特菌知识,为评估冷链中食品和冷藏食品及食品加工设备的安全性提供了理论基础,对冷藏食品使用抗菌剂提出更高要求。

Inactivation effect of acidic electrolyzed water on Listeria monocytogenes under low temperature conditions

Listeria monocytogenes is a psychrophilic bacterium that can grow at low temperatures. The growth of L. monocytogenes in refrigerated ready-to-eat food and frozen food processing equipment is a major food safety issue. Acidic electrolyzed water (AEW) is a new type of high-efficiency, safe and no-residue non-heat sterilization technology. AEW is used to inactivate Listeria monocytogenes in planktonic state, biofilm state, and frozen Flammulina velutipes samples under low temperature conditions. Through the AEW treatment before and after the change of the total number of colonies, biofilm, biofilm structure parameters, virulence gene expression, further analysis of sample experiments, etc. The results showed that when the electrolyte concentration was 0.1% and the treatment time was 1 min, the total number of Listeria monocytogenes colonies and the biofilm clearance rate at 4°C and 37°C were extremely different. The results of this study enrich our knowledge of AEW inactivation of Listeria monocytogenes under low temperature conditions, and provide a theoretical basis for evaluating the safety of food and refrigerated food and food processing equipment in the cold chain. The proposal of antibacterial agents is more demanding.