| 化州橘红种质资源的SSR标记分析 |
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| 引用本文： | 谭婉菁,郑锦铃,简嘉棋,刁金花,黎思娜,李润唐,李映志. 化州橘红种质资源的SSR标记分析[J]. 福建果树, 2011, 0(1): 7-10 |
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| 作者姓名： | 谭婉菁 郑锦铃 简嘉棋 刁金花 黎思娜 李润唐 李映志 |
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| 作者单位： | 广东海洋大学农学院; |
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| 基金项目： | 广东省农业科技推广专项(2008417); 广东省农业科技攻关项目(2008B020200010); 广东省高等学校本科特色专业建设点(2010189); 广东海洋大学大学生创新性实验项目(CXSY200916) |
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| 摘 要： | 利用核基因组简单序列重复（Simple sequence repeat,SSR）DNA标记,对11份化州橘红及其相关种质的遗传多样性进行了研究。结果表明：6对SSR分子标记引物共扩增出32个等位基因,每对引物可扩增3～8个等位基因;11份种质在SSR位点上的PIC值介于0.1763到0.4400之间,平均为0.2761。聚类分析表明,在Nei s遗传相似系数0.85处,可将所研究的种质分为6组,其中有5组分布有化州橘红种质,表明化州橘红不同种质间有较大的遗传差异。
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| 关 键 词： | 化州橘红 种质 遗传多样性 SSR标记 |
Genetic Diversity Analysis of Huazhoujuhong Germplasm Based on SSR Molecular Markers |
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| TAN Wan-qing,ZHENG Jing-ling,JIAN Jia-qi,DIAO Jin-hua,LI Si-na,LI Run-tang,LI Ying-zhi. Genetic Diversity Analysis of Huazhoujuhong Germplasm Based on SSR Molecular Markers[J]. Fujian Fruits, 2011, 0(1): 7-10 |
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| Authors: | TAN Wan-qing ZHENG Jing-ling JIAN Jia-qi DIAO Jin-hua LI Si-na LI Run-tang LI Ying-zhi |
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| Affiliation: | TAN Wan-qing ZHENG Jing-ling JIAN Jia-qi DIAO Jin-hua LI Si-na LI Run-tang LI Ying-zhi(Agricultural College of Guangdong Ocean University,Zhanjiang 524088,Guangdong China) |
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| Abstract: | Simple sequence repeat（SSR） DNA molecular markers of nuclear genome was used to study genetic diversity among 11 Huazhongjuhong accessions and related germplasms.Six pairs of SSR primers amplified 32 alleles,with each pair of primer amplifying 3～8 alleles;PIC values of 11 germplasms in different SSR locus varied from 0.1763 to 0.4400,with an average value of 0.2761.Clustering analysis put 11 germplasms into six groups,among them five groups included Huazhoujuhong.These results indicated that large genetic diversity existed among different Huazhoujuhong germplasm. |
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| Keywords: | Huazhoujuhong Germplasm Genetic diversity SSR marker |
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