柠檬酸、Nisin-柠檬酸联合对三种常见水产病原菌的作用

选用嗜水气单胞菌、副溶血弧菌、荧光假单胞菌三种常见水产病原菌,用于测定柠檬酸的抑菌效果及最小抑菌浓度,并研究了柠檬酸抑菌作用的稳定性。三种菌均为革兰氏阴性菌。试验结果表明,柠檬酸对三种菌都有不同程度的抑菌作用,并得到最小抑菌浓度,依次是:0.0011g/mL,0.0008g/mL,0.00125g/mL;在最小浓度下抑菌作用可达6d之久,柠檬酸抑菌效果有一定的稳定性。将Nisin溶于0.00125g/mL,得到复合液的抑菌效果强于柠檬酸单独作用,且稳定性增强。

Effects of Citric Acid and combined citric acid and nisin on three common pathogenic bacteria in aquaculture

Effect of inhibiting bacteria and Minimum Inhibitory Concentrations(MICs) were determined for citric acid for aeromonas hydrophila,vibrio parahaemolyticus and pseudomonas fluorescens,which of three bacteria being species of aquatic pathogenic bacteria,including durative of inhibiting bacteria.All is G~ bacteria.The results indicate that citric acid for three bacteria are inhibitable.MICs for aeromonas hydrophila,vibrio parahaemolyticus and pseudomonas fluorescens respectively are 0.0011g/ml,0.0008g/ml,0.00125g/ml.Durative may be five days,and the effect is quit permanence.When Nisin dissolve in 0.00125g/ml citric acid,effect of inhibiting is better than citric acid alone,and stableness is also better.