| 大麦芽阿魏酸酯酶的分离纯化及其部分酶学性质的测定 |
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| 引用本文： | 刘莎莎,李华,石庆华,王希东,程发祥.大麦芽阿魏酸酯酶的分离纯化及其部分酶学性质的测定[J].中国畜牧兽医,2010,37(1):28-31. |
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| 作者姓名： | 刘莎莎 李华 石庆华 王希东 程发祥 |
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| 作者单位： | （1.新疆农业大学农学院， 乌鲁木齐 830052； 2.中国农业大学(烟台)， 烟台 2646701；3.新疆农业大学动物科学学院动物营养实验室， 乌鲁木齐 830052） |
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| 基金项目： | 教育部高等学校博士学科点基金项目(20060758003) |
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| 摘 要： | 本试验采用硫酸铵分级沉淀、透析、DEAE-52离子交换层析对大麦芽中的阿魏酸酯酶(FAE)进行了分离纯化,并测定了其部分酶学性质。结果显示,分离纯化后获得了阿魏酸酯酶纯品,纯化倍数达34倍;经SDS-PAGE电泳显示为单一条带,并测定其相对分子质量约为29.3 ku;最适反应温度为50℃;最适pH为5.5,但阿魏酸酯酶在60℃以下,pH 4.5~7.5之间有较好的稳定性;Zn2+、Cu2+、Fe3+对酶活有很强的抑制作用,Na+和EDTA对酶有一定的激活作用。
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| 关 键 词： | 大麦芽 阿魏酸酯酶 分离纯化 酶学性质 |
Purification and Partial Characterisation of Feruloyl Esterase from Barley Malt |
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| LIU Sha-sha,LI Hua,SHI Qing-hua,WANG Xi-dong,CHENG Fa-xiang.Purification and Partial Characterisation of Feruloyl Esterase from Barley Malt[J].China Animal Husbandry & Veterinary Medicine,2010,37(1):28-31. |
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| Authors: | LIU Sha-sha LI Hua SHI Qing-hua WANG Xi-dong CHENG Fa-xiang |
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| Institution: | (1.College of Agronomy Xinjiang Agricultural University, Urumqi 830052,China; 2.China Agricultural University（Yantai）,Yantai 264670, China; 3.Laboratory of Animal Nutrition, College of Animal Science, Xinjiang Agricultural University, Urumqi 830052, China) |
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| Abstract: | The activity of feruloyl esterase has been enhanced in barley grain and barley malt.Feruloyl esterase solution was extracted from barley malt has been purified by ammonium sulfate precipitation，DEAE-cellulose column chromatography.The feruloyl esterase was purified 34-fold as shown in SDS-PAGE gel electrophoresis and the molecular weight estimated to be about 29.3 kD.Characterization of the enzyme exhibited that the optimum pH and the optimum temperature were 5.5 and 50 ℃，respectively，and it lost little activity after one hour below 60 ℃ from pH 4.5 to 7.5.It was inhibited by Zn2+，Fe3+，Cu2+，and strongly activated by EDTA and Na+. |
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| Keywords: | barley malt feruloyl esterase isolation and purification enzyme characterisation |
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