羊副结核性腹泻病病原体分离及药敏试验

为了诊断与治疗阿拉善盟阿左旗发生的羊腹泻病,试验对从阿拉善盟阿左旗采集的病料进行病原体分离培养、抗酸染色、革兰染色、ELISA检测,并对分离到的菌株进行药敏试验。结果表明:从肠系膜淋巴结分离到抗酸染色阳性、革兰染色阳性的短粗杆状副结核杆菌,2份血清中均有与副结核杆菌抗原结合的相应抗体的存在;分离菌对环丙沙星和卡那霉素敏感,对头孢呋肟和阿奇霉素中度敏感,对其他药物均产生不同程度的耐药性。

Isolated of pathogens from the paratuberculosis of sheep diarrhea and drug susceptibility tests

To diagnose and treat the sheep diarrhea in Alashanzuoqi,the collection of materials from Alashanzuoqi was used to isolate and cultured the pathogens,then the methods of acid-fast stain,gram stain,ELISA testing and drug susceptibility test were used to detect the strains.The results showed that the strains were identified as Mycobacterium paratuberculosis with short and rod shaped,separated from the mesenteric lymph node and showed positive by acid-fast stain and Gram strain.The corresponding antibodies with Mycobacterium paratuberculosis antigen existed in the two serums.The drug susceptibility test indicated the isolated bacteria was sensitive to ciprofloxacin and kanamycin,and moderately sensitive to cefuroxime and azithromycin,it had different levels of drug resistance to the other medicines.