| 人参水溶性蛋白的纯化工艺研究 |
| |
| 引用本文： | 张巍,李红艳,马晶,姜先刚,赵雨,赵大庆. 人参水溶性蛋白的纯化工艺研究[J]. 吉林农业大学学报, 2008, 30(1): 36-39 |
| |
| 作者姓名： | 张巍 李红艳 马晶 姜先刚 赵雨 赵大庆 |
| |
| 作者单位： | 长春中医药大学中医药与生物工程研发中心,长春,130117 |
| |
| 基金项目： | 科技部“十五”攻关项目(2004BA907A17) |
| |
| 摘 要： | 为研究人参中几种水溶性蛋白的提取纯化工艺,采用中性缓冲液抽提和硫酸铵分级沉淀法提取人参总蛋白,应用超滤、亲和层析、离子交换层析、疏水层析、凝胶过滤层析等方法进行分离、纯化,得到5种水溶性人参蛋白。经SDS-PAGE电泳和高效凝胶过滤色谱分析,其分子量分别为65.8,65.5,24.7,15.1,7.6 kD。研究结果表明,本试验所确定的人参水溶性蛋白纯化工艺路线简便、可行。
|
| 关 键 词： | 人参 水溶性蛋白 纯化 |
| 文章编号： | 1000-5684(2008)01-0036-04 |
| 修稿时间： | 2007-02-14 |
Purification of several ginseng Proteins |
| |
| ZHENG Wei,LI Hong-yan,MA Jing,JIANG Xian-gang,ZHAO Yu,ZHAO Da-qing. Purification of several ginseng Proteins[J]. Journal of Jilin Agricultural University, 2008, 30(1): 36-39 |
| |
| Authors: | ZHENG Wei LI Hong-yan MA Jing JIANG Xian-gang ZHAO Yu ZHAO Da-qing |
| |
| Abstract: | The purification progress of several ginseng proteins was studied.The hydrophilic proteins of ginseng were purified by means of salt solution extraction,ammonium sulfate fractionation,ultrafiltration, affinity chromatography on Affi-Gel blue gel,ion exchange chromatography on DEAE-Sepharose and SP-Sepharose,hydrophobic interaction chromatography on Phenyl-Sepharose.Five ginseng proteins were obtained.Their purities were analysed by SDS-polyacrylamide gels electrophoresis and their molecular weights were determined to be 65.8,65.5,24.7,15.1,7.6 kD,respectively,by Gel Filtration Chromatography.This method is simple and feasible. |
| |
| Keywords: | ginseng hydrophilic protein purification |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
