高CO2浓度对春兰叶片叶绿素·叶绿素蛋白质复合物含量的影响

[目的]为了研究春兰适应环境变化的方法。[方法]对自然状态（正常组,CO2浓度（370±50）μl/L）和高CO2浓度（试验组,CO2浓度（700±50）μl/L）春兰叶片的叶绿素及叶绿素蛋白质复合物含量进行研究。[结果]与正常组相比,试验组春兰叶片的叶绿素含量增高,叶绿素a/b降低;叶绿素蛋白质复合物的含量增加,经SDS-聚丙烯酰胺凝胶电泳得到6条叶绿素蛋白质复合物带和1条游离色素带,表明叶绿素蛋白质复合物种类未发生变化。从电泳结果可以看出,叶绿素蛋白质复合物聚合态如LHCⅡ3的量增多,单体态LHCⅡ1和LHCⅡ2的量减少。[结论]叶绿素、叶绿素蛋白质复合物含量的变化是春兰对高CO2浓度的一种适应效应,有利于提高其在光合作用中光能的吸收、传递和转换的效率,并且支持高效的光合碳素同化作用。

Effects of CO_2 Doubling on Leaf Chlorophyll and Chlorophyll-Protein Complexes in Cymbidium goeringii Rchb.f.

[Objective]The research aimed to study the method of Cymbidium goeringil adapting to environmental change.[Method] The effects of high CO2 concentration on the chlorophyll and chlorophyll-protein complexes of Cymbidium goeringii leaves were studied.[Result]Compared with the normal CO2 concentration(370±50 μl/L),the contents of chlorophyll and chlorophyll-protein increased at high CO2 concentration(700±50 μl/L).The contents of polymer state of PSⅡlight-harvesting chlorophyll a/b-protein complex(LHCⅡ) increased as the monomer state of LHCⅡdecreased.The changes of the contents of chlorophyll and chlorophyll-protein,and the state of LHCⅡin Cymbidium goeringii were an adaptive effects of plant photosynthetic mechanism to the long term elevated CO2.[Conclusion] These changes increased the efficiency of the absorption,transfer and conversion of light energy in plant photosynthesis,and support the high efficiency of photosynthetic carbon assimilation.