十五个产地兴安落叶松种子同工酶的研究

本实验应用聚丙烯酰胺凝胶电泳，对１５个不同产地的兴安落叶松种子进行酯酶（ＥＳＴ）、苹果酸脱氢酶（ＭＤＨ）和过氧化物酶（ＰＯＸ）同工酶的分离。结果表明，兴安落叶松种子ＥＳＴ、ＭＤＨ和ＰＯＸ同工酶谱均存在３种类型，这是该树种在进货的历史长河中对环境的适应，且在分子水平上的表现。在用同工酶法鉴别该树种时，应考虑因产地不同而可能引起同工酶谱的差异。

STUDIES ON THE ISOZYMES OF Larix gmelini Rupr.SEEDS FROM FIFTEENPLACES

In this experiment the EST.MDH and POX in the seeds of Larix gmelini Rupr.from fifteen different places have been separated by means of polyacrylamide gel elec-trophoresis,The results show that the zymograms of EST,MDH and POX in the seeds ofkarix gmelinii, Rupr,have three types respectively. It is the results of adaptation to the envi-ronment and of expression in molecular level. The differences of the zymograms caused byenvironmental differences should be considered when the seeds of the trees are identified bythe means of isozymes.