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| 白腐菌TP21液体培养产漆酶条件的研究 | |
| 引用本文： | 苏东海,苏东民,辛秀兰,孙君社.白腐菌TP21液体培养产漆酶条件的研究[J].西北农业学报,2009,18(3):249-253. |
| 作者姓名： | 苏东海 苏东民 辛秀兰 孙君社 |
| 作者单位： | 1. 北京电子科技职业学院,生物系,北京,100029 2. 河南工业大学,河南郑州,450001 3. 中国农业大学,食品科学与营养工程学院,北京,100083 |
| 基金项目： | 北京市属市管高等学校人才强教项目资助项目 |
| 摘 要： | 研究了碳源、氮源、各种理化因素以及培养条件对白腐菌TP21漆酶分泌的影响.结果表明:木糖和多糖作碳源、酵母膏作氮源有利于漆酶的分泌,pH在4.0～6.0的范围内对漆酶的分泌影响不大,培养温度、接种量、通气量对漆酶的分泌有较大影响,培养基中添加诱导物对漆酶的产生有促进作用,特别是玉米秸秆价格低廉,是较好的选择. |
| 关 键 词： | 白腐菌 漆酶 培养条件 |
| 收稿时间： | 2008/9/28 0:00:00 |
| 修稿时间： | 2008/11/16 0:00:00 |
Research on Laccase Production by White-rot Fungus TP21 in Submerged Culture |
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| SU Donghai,SU Dongmin,XIN Xiulan and SUN Junshe.Research on Laccase Production by White-rot Fungus TP21 in Submerged Culture[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(3):249-253. | |
| Authors: | SU Donghai SU Dongmin XIN Xiulan and SUN Junshe |
| Institution: | Beijing Vocational College of Electronic Science, Beijing 100029, China;He''nan University of Technology, Zhengzhou 450001, China;Beijing Vocational College of Electronic Science, Beijing 100029, China;China Agricultural University, Beijing 100083, China |
| Abstract: | The effect of carbon source/nitrogen source/physicochemical element and culture condition on lactase production of white-rot fungus TP21 was studied.The results showed that starch and yeast paste were better to be used as carbon source and asnitrogen source respectively.There was little difference of effect of pH 4.0~6.0 of the medium on lactase production,but the culture temperature,inoculation amount and ventilation volume had greater influence on lactase production by the fungus.The curveof lactase production and optimal condition of lac case reaction were also described.Incubation temperature,inoculum concentration,minute ventilation volume have great effect on lactase production.Additive inducer in culture medium has the promoter action to lac case production.Especially those maize straws are inexpensive ingredients which be selected. |
| Keywords: | White-rot fungus Laccase Culture conditions |
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