市售鲜猪肉细菌的分离鉴定及其耐药性分析

为了解市售鲜猪肉的细菌污染状况及主要病原菌的耐药性,以便从源头上控制市售鲜猪肉的安全,甚至为细菌引发的食源性疾病的治疗提供参考依据,从遵义和安顺地区农贸市场采集24份鲜猪肉样本进行细菌分离鉴定及药敏试验。结果表明:鲜猪肉表层与深部细菌含量分别在(1.87～7.13)×105cfu/g和(1.0～1.3)×104cfu/g,分离鉴定的细菌有葡萄球菌、大肠杆菌、链球菌、芽孢杆菌、塔特姆氏菌、耶尔森氏菌、沙雷氏菌、不动杆菌、莫拉氏菌和假单胞菌。大肠杆菌和葡萄球菌均存在广泛的耐药性,对麦迪霉素、淋必治、氯洁霉素、灭滴灵、阿奇霉素、吡哌酸、洁霉素、复方新诺明、罗红霉素和先锋霉素IV的耐药性达100%。

Isolation and Identification of Pathogens in Fresh Pork and Its Drug Sensitivity Test

To grasp the contamination and the resistance of pathogenic bacteria in fresh pork,control the safety of fresh pork at the source and to provide references for cure of food origin disease caused by bacteria.24 pork samples were collected for bacteriological isolation and identification and drug sensitivity.The results showed that the number of bacteria in surface and deep pork were respectively(1.87~7.13)×105 cfu/g and(1.0~1.3)×104 cfu/g.And they were mainly Staphylococcus,Streptococcus,Bacillus,Escherichia coli,Tatumella,Yersinia,Serratia,Acinetobacter,Moraxella and Pseudomonas.The lowest sensitive drug for Staphylococcus and E.coli were midecamycin,trobicin,clindamycin,metronidazole,azithromycin,pipemidic acid,jiemycin,sinomin,roxithromycin,cephaloridine IV,the drug resistance was up to 100%.