陆川猪种群线粒体DNA遗传多样性研究

利用线粒体控制区(mtDNA D-loop)5’端564 bp的片段,对6个陆川猪种群共74个个体的遗传多样性进行研究。在74个序列中共发现20个变异位点,产生7个单倍型,其中1个为优势单倍型。总的单倍型多样性和核苷酸多样性指数分别为0.530和0.00326,表明陆川猪种群遗传多样性偏低。6个种群中,仁厚镇福绵种群的单倍型多样性和核苷酸多样性较高,乌石镇种群单倍型和核苷酸多样性均较低。将优势单倍型H1与已报道的其他21个中国地方猪种的mtDNA D-loop序列进行系统进化分析,结果显示陆川猪和属于华中型的宁乡猪、大花白猪有更近的亲缘关系,但与传统上同属华南型的香猪和滇南小耳猪的遗传关系较远。

Genetic diversity of Luchuan pig inferred from the mitochondrial DNA control region

In order to know genetic diversity of some captive populations and provide useful information for conservation and management of Luchuan pig,564 bp fragment at the 5’-end of the mitochondrial DNA control region were assessed for 74 individuals from six captive populations in present study.Twenty variable sites and 7 haplotypes were observed in 74 individuals,and the haplotype and nucleotide diversities(h=0.530 and π=0.00326) were relatively low compared with those of other captived mammals.In the six investigated populations,the Fumian population had the highest nucleotide diversity and haplotype diversity,the Wushi population had the lowest.The overall mean intra-population genetic distance of the Fumian population was larger than that of the other five populations.The phylogenetic tree of Luchuan pig and other 21 species showed that the Luchuan pig shared a closer genetic relationship with Ningxiang pig and Dahuabai pig than Xiang pig and Diannanxiaoer pig.