| 樟芝发酵产2-苯乙醇的条件优化 |
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| 引用本文： | 贾薇,白岩岩,冯杰,张劲松,贾新成.樟芝发酵产2-苯乙醇的条件优化[J].食用菌学报,2016(1):47-52. |
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| 作者姓名： | 贾薇 白岩岩 冯杰 张劲松 贾新成 |
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| 作者单位： | 1. 上海市农业科学院食用菌研究所，农业部南方食用菌资源利用重点实验室，国家食用菌工程技术研究中心，国家食用菌加工技术研发分中心，上海市农业遗传育种重点开放实验室，上海 201403;2. 河南农业大学生命科学学院,河南郑州,450002 |
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| 基金项目： | 上海市科技兴农重点攻关项目[沪农科攻字(2012)第2-9号]资助 |
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| 摘 要： | 以药用真菌樟芝(Taiwanofungus camphoratus)发酵液中的主要香气成分之一2-苯乙醇的含量为指标,利用单因素和正交试验对发酵条件进行优化,获得了樟芝发酵产2-苯乙醇的最优条件：培养基(g/L)为50葡萄糖,5酵母粉,1磷酸氢二钾,0.5硫酸镁,0.1 VB1,pH 为5；装液量为120 mL/250 mL 三角瓶；接种量20%；转速150 r/min；26℃恒温培养7 d；在此条件下樟芝发酵液中的2-苯乙醇含量达到1.24 g/L。
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| 关 键 词： | 樟芝 发酵 2-苯乙醇 条件优化 |
Optimization of Selected Growth Parameters Affecting 2-Phenylethanol Production in Submerged Cultures of Taiwanofungus camphoratus |
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| Abstract: | Selected culture parameters affecting the production of 2-phenylethanol (2-PE ), an aroma compound with a rose-like fragrance that is widely used in the cosmetic,perfume,and food industries,in submerged cultures of Taiwanofungus camphoratus grown in 250 mL flasks have been investigated.Single factorial experiments and orthogonal testing revealed that 2-PE synthesis was maximal (1 .24 g/L)when the fungus was grown in a culture medium consisting of (g/L)50 glucose,5 yeast powder,1 K2 HPO4 ,0.5 MgSO4 and 0.1 vitamin B1 ,and when the following conditions were applied:initial pH of the medium,pH 5;volume of medium per 250 mL flask,120 mL;inoculum size,20%;flask rotation speed,150 r/min;culture temperature,26 ℃;culture time,7 d. |
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| Keywords: | Taiwanofungus camphoratus submerged culture 2-phenylethanol optimization of growth conditions |
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