膜过滤法分离纯化刺芹侧耳提取液中海藻糖的工艺 |
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引用本文: | 周帅,张忠,冯娜,刘艳芳,唐庆九.膜过滤法分离纯化刺芹侧耳提取液中海藻糖的工艺[J].食用菌学报,2016(1):59-62. |
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作者姓名: | 周帅 张忠 冯娜 刘艳芳 唐庆九 |
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作者单位: | 上海市农业科学院食用菌研究所,农业部南方食用菌资源利用重点实验室,国家食用菌工程技术研究中心,国家食用菌加工技术研发分中心,上海市农业遗传育种重点开放实验室,上海 201403 |
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基金项目: | 国家农业部行业专项(201303080),上海市农业科学院科技发展基金项目[农科发2013(12)]资助 |
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摘 要: | 联合使用截留相对分子质量1000的超滤膜和270的纳滤膜从刺芹侧耳(Pleurotus eryngii)水提液中分离海藻糖。超滤膜操作压力为4 kg/cm2,同时纳滤膜操作压力为3 kg/cm2,经膜分离的相对分子质量270~1000部分溶液经浓缩结晶,海藻糖纯度可达93.3%。
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关 键 词: | 刺芹侧耳 膜过滤 海藻糖 |
Separation of Trehalose from Aqueous Extracts of Pleurotus eryngii Fruit Bodies Using Membrane Filtration |
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Abstract: | Pleurotuseryngii fruit bodies (1 kg)were extracted twice with ddH2O at 100 ℃ for 2 h (fruit body∶water ratio was 1∶15 w/v for the first extraction and 1∶10 w/v for the second).Combined extracts (total volume~18 L)were filtered through cheese cloth and the filtrate was separated into three fractions using a polyether sulphone membrane filter (PES1)(MWCO 1000 Da)and a NF270 nanofilter (MWCO 270 Da). Pressures of 4 kg/cm2 and 3 kg/cm2 were applied when filtering through the PESI and NF270 membranes, respectively.The fraction passing through the PESI membrane but retained by the NF270 nanofilter was concentrated using a rotary evaporator and the trehalose concentration in this fraction was adjusted to 30%. Five volumes (v/v)of absolute ethanol were added and,after mixing,the solution was left to stand overnight at 4 ℃.Precipitated material was collected by suction filtration and re-crystallized from water∶absolute ethanol (1∶5,v/v)to give a trehalose sample of 93.3% purity. |
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Keywords: | Pleurotus eryngii membrane filtration trehalose |
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