尖顶羊肚菌多糖的超声波辅助提取工艺优化及其对肝损伤小鼠抗氧化活性的影响

采用正交试验优化超声波辅助提取尖顶羊肚菌(Morchella conica)多糖工艺,并分析多糖对肝损伤小鼠体内抗氧化活性.结果表明,在超声功率600 W、提取温度60℃、提取时间80 min、醇沉浓度70％、料液比1∶40(g∶mL)的条件下,多糖得率为(2.25±0.04)％.影响多糖得率的因素由大到小依次为超声功率、提取温度、料液比、乙醇浓度和提取时间.与CCl4诱导的小鼠急性肝损伤模型组相比,灌胃尖顶羊肚菌多糖处理组的肝组织中丙二醛含量显著降低、总抗氧化能力显著提高;血清中丙二醛含量显著降低、超氧化物歧化酶活性和总抗氧化能力显著增加.

Ultrasound-assisted Extraction of Polysaccharides from Morchella conica and Their Effects on Selected Antioxidant Indices in Mice with CCl4-induced Liver Injury

Orthogonal testing was used to optimize selected parameters affecting ultrasound-assisted aqueous extraction of polysaccharides from Morchella conica fruit bodies.Polysaccharide yields were highest (2.25 ± 0.04) ％ using an ultrasound power setting of 600 W,an extraction temperature of 60 ℃,80 min extraction time,precipitation using a final ethanol concentration of 70％,and a fruit body:extraction solvent ratio of 1∶40 (g∶ mL).The selected parameters influenced polysaccharide yields in the order ultrasound power setting＞ extraction temperature＞ fruit body:extraction solvent ratio＞final ethanol concentration＞extraction time.Malondialdehyde levels were significantly lower,and total antioxidant capacity was significantly higher,in both the sera and liver tissue of polysaccharide-treated mice compared with mice with CCl4-induced liver injury.Significantly higher levels of superoxide dismutase activity were also recorded in polysaccharidetreated mice.