| 一种测定土壤反硝化酶的改进方法 |
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| 引用本文： | 韩建刚,白红英,朱咏莉,李世清,曲东.一种测定土壤反硝化酶的改进方法[J].农业环境科学学报,2002,21(4):349-351. |
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| 作者姓名： | 韩建刚 白红英 朱咏莉 李世清 曲东 |
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| 作者单位： | 西北农林科技大学资源与环境科学院,陕西杨凌,712100 |
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| 基金项目： | 国家自然科学基金部分资助(39970151) |
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| 摘 要： | 介绍了一种新的测定土壤反硝化酶的装置,使土壤反硝化酶(硝酸还原酶、亚硝酸还原酶)的测定更加简便、灵敏、准确。对3种不同质地的土壤从影响土壤反硝化酶活性的3种关键因素:培养时间、称样量和真空度方面进行了研究。结果表明,土壤反硝化酶的最佳培养时间为24—27h,称样量与真空度因酶而异。
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| 关 键 词： | 硝酸还原酶 亚硝酸还原酶 培养时间 称样量 真空度 |
| 文章编号： | 1000-0267(2002)04-0349-03 |
| 修稿时间： | 2001年8月7日 |
An Improved Method for Determination of Denitrifying Enzymes in Soil |
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| HAN Jian-gang,BAI Hong-ying,ZHU Yo ng-li,LI Shi-qing,QU Dong.An Improved Method for Determination of Denitrifying Enzymes in Soil[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2002,21(4):349-351. |
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| Authors: | HAN Jian-gang BAI Hong-ying ZHU Yo ng-li LI Shi-qing QU Dong |
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| Abstract: | A simple and improved method for determination of denitrifying enzymes(nitrate reductase and nitrite reductase)in soil.In comparison with the previous meth ods,it is characterized by more simp le,sensitive and accurate.Three affecting factors including incubation period,sample weight an d vacuum have been optimized through selection of three soils.It has been indicated that the optical incubation period time was 24-27hours,but sample weight and vacuum for the test were variable based on e nzames. |
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| Keywords: | Nitrate reductase nitrite reductase sample weight incubation period vacuum |
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