小麦 TaSPL17 基因在苗期对不同激素处理的响应

小麦TaSPL17基因在小麦株型调控中具有重要作用。为发掘TaSPL17基因在小麦育种中的应用价值,本研究采用不同浓度的独脚金内酯人工合成类似物GR24、生长素IAA和细胞分裂素6-BA分别处理三叶期的小麦,通过qRT-PCR技术检测小麦苗期根部和茎基部TaSPL17基因相对表达量的变化。结果发现,外源施加GR24、IAA和6-BA能显著下调小麦幼苗根部和茎基部TaSPL17基因的表达,并且在一定范围内施加的激素浓度越高,对TaSPL17基因表达的抑制作用越明显。以上结果表明,小麦株型相关基因TaSPL17的表达量可以被独脚金内酯、生长素和细胞分裂素调节,该基因可能通过参与这三种激素的信号途径发挥其在株型调控中的作用。

Response of TaSPL17 Gene to Different Hormone Treatments in Wheat Seedlings

TaSPL17 plays an important role in the regulation of wheat plant architecture. In order to explore the molecular mechanism of TaSPL17 regulating wheat growth and development, and study the application value of TaSPL17 gene in wheat breeding,different concentrations of strigolactone synthetic analogues GR24, auxin IAA and cytokinin 6-BA were used to treat the three-leaf stage wheat, and the qRT-PCR technique was used to detect the relative expression level of TaSPL17 in wheat root and stem base in this study. It was found that the application of GR24, IAA and 6-BA significantly inhibited the expression of TaSPL17 gene in the root and stem base of wheat seedlings, and the inhibition of TaSPL17 was increased with the improvement of hormone concentrations.The above results showed that TaSPL17 gene related to wheat architecture can be regulated by strigolactone,auxin and cytokinin,and this gene could play its role in plant architecture regulation through participating in these three hormone signaling pathways.