Systemic aspects of host–pathogen interactions in Austrian pine (Pinus nigra): A proteomics approach |
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Authors: | Duan Wang Alieta Eyles David Mandich Pierluigi Bonello |
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Affiliation: | aDepartment of Plant Pathology, The Ohio State University, Columbus, OH 43210, USA;bPlant-Microbe Genomic Facility, The Ohio State University, Columbus, OH 43210, USA |
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Abstract: | The molecular basis of systemic resistance induced in Austrian pine by two canker pathogens was investigated using a proteomics approach. Protein from phloem located 50 cm above the induction site was extracted 26 days after inoculation. Nineteen proteins shown to be up/down-regulated by the pathogens were selected for sequencing. Fifteen proteins were assigned putative functions, revealing that several represented small heat shock proteins. No PR-proteins were identified among the others, which included a proteasome endopeptidase, two oxygen-evolving enhancer proteins, a nucleoside diphosphate kinase, a fructose bisphosphaste aldolase, a ribulose bisphosphate carboxylase, a phosphoserine aminotransferase, and a formate dehydrogenase. |
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Keywords: | Austrian pine S. sapinea D. scrobiculata Systemic-induced resistance 2-D gel electrophoresis Small heat shock proteins |
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