Influence of the cumulus and gonadotropins on the metabolic profile of porcine cumulus-oocyte complexes during in vitro maturation |
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| Authors: | Gm Alvarez Gc Dalvit Pd Cetica |
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| Affiliation: | Institute of Research and Technology in Animal Reproduction (INITRA), Area of Biochemistry, School of Veterinary Sciences, University of Buenos Aires, Buenos Aires, Argentina. |
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| Abstract: | The aim of this work was to examine the influence of the cumulus and gonadotropins on the metabolic profile of porcine cumulus oocyte complexes (COCs) during in vitro maturation. Immature COCs were assigned to morphological classes A(1) (with a dense cumulus), A(2) (with a translucent cumulus), B(1) (with the corona radiata), B(2) (with only some remaining cumulus cells) and matured with or without gonadotropins. Glycolysis and ammonia production were higher in the A class COCs; gonadotropins increased both, especially in the A(1) COCs (p?
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