Flow cytometric enumeration of reticulocyte in the peripheral blood from canine infected with Babesia gibsoni |
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| Authors: | T Uemura S Suzuki T Ohnishi |
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| Institution: | Department of Veterinary Science, College of Agriculture, University of Osaka Prefecture, Japan. |
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| Abstract: | Peripheral blood samples from dogs infected with Babesia gibsoni were analyzed by a flow cytometer for the percentage of reticulocytes after staining with a membrane-permeable fluorochrome, thiazole orange. Though thiazole orange has been reported to stain human reticulocytes and Plasmodium-infected erythrocytes, number of positive cells determined by the flow cytometry did not include that of erythrocytes infected with Babesia gibsoni. Analysis of 51 samples revealed a correlation coefficient of 0.96 as compared to the conventional determination by light microscopy. Separation of reticulocytes from Babesia gibsoni-infected erythrocytes by flow cytometry with or without the stain remained unresolved. |
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