中华蜜蜂工蜂碱性磷酸酶功能基团的研究

在一定条件下，分别采用二硫代二硝基苯甲酸（DTNB）、N-溴代琥珀酰亚胺（NBS）、苯甲基磺酰氟（PMSF）、溴乙酸（BrAc）、乙酰丙酮、巯基乙醇（ME）等化学修饰剂选择修饰中华蜜蜂碱性磷酸酶多种氨基酸残基，并测定其酶活力变化。结果表明，DTNB、PMSF的修饰不表现对酶的抑制作用，而NBS、BrAc、ME能显著抑制酶的活力，乙酰丙酮也能抑制酶的活力，但是不太明显。因此认为Trp、His是中华蜜蜂工蜂碱性磷酸酶的必需功能基团，部分二硫键对保护酶的催化功能也是必需的。

Functional Groups of Alkaline Phosphatase from Apis cerana cerana Fabricius

The alkaline phosphatase was selectively modified by DTNB,NBS,PMSF,BrAc,acetyl acetone and ME, and changes in the activities of the enzyme have been detected. The reaction of alkaline phosphatase with NBS ,BrAc and ME resulted in a strong inhibition of the enzyme activities which decreased with the increase of modifier concentration. The DTNB,PMSF were found without any inhibition effect. The acetyl acetone was found to have little inhibition effect on AKP activity. All of these suggested that Trp and His residues should considered as indispensable functional groups of AKP, and partial disulfide bonds as essential for the function of the enzyme.