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| 瘤胃微生物基因组文库中BAC末端序列分析 | |
| 引用本文： | 赵圣国,王加启,李发弟,刘开朗,卜登攀,李旦,于萍.瘤胃微生物基因组文库中BAC末端序列分析[J].甘肃农业大学学报,2010,45(2). |
| 作者姓名： | 赵圣国 王加启 李发弟 刘开朗 卜登攀 李旦 于萍 |
| 作者单位： | 1. 甘肃农业大学动物科学技术学院,甘肃,兰州,730070;中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京,100193 2. 甘肃农业大学动物科学技术学院,甘肃,兰州,730070 3. 中国农业科学院北京畜牧兽医研究所,动物营养学国家重点实验室,北京,100193 |
| 基金项目： | 国家科技支撑计划(2006BAD04A03);;动物营养学国家重点实验室自主研究课题(2004DA125184(团)0801) |
| 摘 要： | 基于前期从瘤胃微生物基因组文库中筛选的功能酶克隆菌,利用T7和pIBRP引物,测定功能酶克隆菌的BAC末端序列,并利用NCBI Blast系统对BAC末端序列进行分析.结果表明:共得到26条BAC末端序列,经Blast比对分析,与已知编码基因的匹配度低,而大部分BAC末端序列与阪崎肠杆菌和环境中的微生物匹配度高,说明瘤胃中的微生物与其它环境中的微生物具有一定的相似性,进一步丰富了对瘤胃微生物的认识. |
| 关 键 词： | 瘤胃微生物 宏基因组文库 BAC末端序列 Blast比对 |
Analysis of bacterial artificial chromosome (BAC) end sequences from microbial metagenomic library of rumen |
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| ZHAO Sheng-guo,WANG Jia-qi,LI Fa-di,LIU Kai-lang,BU Deng-pan,LI Dan,YU Ping.Analysis of bacterial artificial chromosome (BAC) end sequences from microbial metagenomic library of rumen[J].Journal of Gansu Agricultural University,2010,45(2). | |
| Authors: | ZHAO Sheng-guo WANG Jia-qi LI Fa-di LIU Kai-lang BU Deng-pan LI Dan YU Ping |
| Institution: | 1.College of Animal Science and Technology;Gansu Agricultural University;Lanzhou 730070;China;2.State Key Lab of Animal Nutrition;Institute of Animal Science;Chinese Academy of Agricultural Sciences;Beijing 100193;China |
| Abstract: | The bacterial artificial chromosome(BAC) end sequences of enzyme positive clones screened from the microbial metagenomic library of rumen were sequenced using the primers of T7 and pIBRP.Then the BAC end sequences were blasted to NCBI database.The results revealed that 26 BAC end sequences were attained.These sequences had poor identity to known encoding genes,and had the best identity to Enterobacter sakazakii and environmental uncultured bacterial.Rumen contained some microbes similar to other environment... |
| Keywords: | rumen microflora metagenome BAC end sequences blast analysis |
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