| 亚洲棉石系亚1号耐旱相关基因SSH文库的构建及其分析 |
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| 引用本文： | 张玲,李付广,刘传亮,张朝军,武芝霞.亚洲棉石系亚1号耐旱相关基因SSH文库的构建及其分析[J].棉花学报,2010,22(2):110-114. |
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| 作者姓名： | 张玲 李付广 刘传亮 张朝军 武芝霞 |
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| 作者单位： | 中国农业科学院棉花研究所/农业部棉花遗传改良重点实验室,河南,安阳,455000 |
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| 基金项目： | 国家“863”计划优质高产棉花分子品种的创制(2006AA100105) |
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| 摘 要： | 通过抑制消减杂交技术成功地构建了亚洲棉石系亚1号耐旱相关基因cDNA文库。通过蓝白斑筛选、菌落PCR及生物信息学等分子生物学手段分析和检验了库的质量,最终筛选出阳性克隆392个。通过EST测序得到265个单一序列,其中41个为重叠群,224个为单拷贝。生物信息学分析结果表明,该文库含有大量与耐旱相关的基因,并且涉及植物生理中多种代谢途径。
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| 关 键 词： | 亚洲棉 干旱胁迫 抑制性消减杂交 |
| 收稿时间： | 2008-11-04; |
Isolation and Analysis of Drought-related Gene from Cotton (Gossypium arboreum L.)SSH Library |
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| ZHANG Ling,LI Fu-guang,LIU Chuan-liang,ZHANG Chao-jun,WU Zhi-xia.Isolation and Analysis of Drought-related Gene from Cotton (Gossypium arboreum L.)SSH Library[J].Cotton Science,2010,22(2):110-114. |
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| Authors: | ZHANG Ling LI Fu-guang LIU Chuan-liang ZHANG Chao-jun WU Zhi-xia |
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| Institution: | Cotton Research Institute/a>;Chinese Academy of Agriculture Sciences/a>;Key Laboratory of Cotton Genetic Improvement/a>;Ministry of Agriculture/a>;Anyang/a>;Henan 455000/a>;China |
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| Abstract: | This study constructed high quality drought-related cDNA library of cotton (Gossypium arboreum L.) by SSH successfully;it was checked and analyzed by Blue-white bolting,colony PCR,and bioinformatics.Finally,392 positive clones were obtained.After cluster analysis of the ESTs sequences,265 unigenes were obtained,including 41 contigs and 224 singlets.A large group of drought stress-induced genes were found in the cDNA library,which involved in many metabolism pathways. |
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| Keywords: | Gossypium arboreum L drought stress SSH |
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