|
|||||
|
|
| 抑制性消减杂交方法克隆棉属突变材料腺体发育相关的cDNAs | |
| 引用本文： | 蔡应繁,莫剑川,曾宇,任薇薇,苗琛,徐莺,王胜华,陈放.抑制性消减杂交方法克隆棉属突变材料腺体发育相关的cDNAs[J].北京林业大学学报,2003,25(3):6-10. |
| 作者姓名： | 蔡应繁 莫剑川 曾宇 任薇薇 苗琛 徐莺 王胜华 陈放 |
| 作者单位： | 1. 四川大学生命科学学院;西南交通大学生物工程系 2. 西南交通大学生物工程系 3. 四川大学生命科学学院 |
| 基金项目： | 四川省青年基金;462; |
| 摘 要： | 该文采用抑制性消减杂交方法 (SSH)构建了棉属突变材料“湘棉 18”种子萌发过程中腺体发育形成前后的两个不同时期的SSH差减文库 .通过差示筛选及反式Northern检测 ,获得了色素腺体形成时期特异表达或优势表达的cDNA片段 .结果表明 ,这些cDNA所涉及的基因 ,多数是棉属中新发现的 ,它们与发育调控、细胞凋亡、蛋白质合成等密切相关 . |
| 关 键 词： | 抑制性消减杂交(SSH) 色素腺体 发育 克隆 棉属 差示筛选 反式Northern |
Cloning of cDNAs associated with the development of pigment gland of Gossypium by suppression subtractive hybridization |
|
| Cai Yingfan, Mo Jianchuan, Zeng Yu, Ren Weiwei, Miao Chen, Xu Ying,Wang Shenghua, Chen Fang.Life College of Sichuan University,Chengdu,P.R.China..Cloning of cDNAs associated with the development of pigment gland of Gossypium by suppression subtractive hybridization[J].Journal of Beijing Forestry University,2003,25(3):6-10. | |
| Authors: | Cai Yingfan Mo Jianchuan Zeng Yu Ren Weiwei Miao Chen Xu Ying Wang Shenghua Chen FangLife College of Sichuan University Chengdu PRChina |
| Institution: | Cai Yingfan, Mo Jianchuan, Zeng Yu, Ren Weiwei, Miao Chen, Xu Ying,Wang Shenghua, Chen Fang.Life College of Sichuan University,Chengdu,610064,P.R.China. |
| Abstract: | Two differentially expressed cDNA libraries of forward and reverse subtraction were constructed using suppression subtractive hybridization (SSH) to isolate the gland related genes differentially expressed in the development of special pigment gland of Gossypium in the process of germinating of the seeds of Xiangmian 18 The authors obtained some cDNA fragments differentially expressed in the time of development of the gland through screening the libraries and virtual northern analysis. The results revealed that the differentially expressed cDNA fragments were associated with regulation and control factors, apoptosis, protein synthesis etc. |
| Keywords: | suppression subtractive hybridization pigment gland development cloning \%Gossypium \% differentially screening virtual northern analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |