| 毛白杨叶际酵母Wapdr15基因的克隆及功能鉴定 |
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| 引用本文： | 郝甜,赵新伟,孙然,才满,杜克久. 毛白杨叶际酵母Wapdr15基因的克隆及功能鉴定[J]. 林业与生态科学, 2021, 36(1) |
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| 作者姓名： | 郝甜 赵新伟 孙然 才满 杜克久 |
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| 作者单位： | 河北农业大学林学院,河北保定071000;河北林木种质资源与森林保护重点实验室,河北保定071000 |
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| 基金项目： | 河北省自然科学基金(C2018204134)。 |
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| 摘 要： | 为探究ABC转运蛋白介导毛白杨叶际异常维克汉姆酵母对BDE-3的耐受机制,通过对其中Wapdr15基因克隆、基因序列及编码蛋白质结构分析、烟草遗传转化以及转Wapdr15基因烟草的BDE-3暴露胁迫实验,发现Wapdr15基因编码序列长4536 bp,编码蛋白具有TMD1-NBD1-TMD2-NBD 2结构,为全分子跨膜蛋白;转Wapdr15基因烟草组培苗叶片对BDE-3的耐受性显著提高,推测Wapdr15参与了毛白杨叶际异常维克汉姆酵母对BDE-3的解毒耐受过程。
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| 关 键 词： | 毛白杨 叶际 异常维克汉姆酵母 ABC转运蛋白 BDE-3 |
Cloning and functional identification of Wapdr15 gene in Wickerhamomyces anomalus isolated from Populus tomentosa Phyllosphere |
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| HAO Tian,ZHAO Xinwei,SUN Ran,CAI Man,DU Kejiu. Cloning and functional identification of Wapdr15 gene in Wickerhamomyces anomalus isolated from Populus tomentosa Phyllosphere[J]. Forestry and Ecological Sciences, 2021, 36(1) |
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| Authors: | HAO Tian ZHAO Xinwei SUN Ran CAI Man DU Kejiu |
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| Affiliation: | (College of Forestry,Hebei Agricultural University,Baoding 071000,China;Key Laboratory of Germplasm Resourse and Forest Protection of Hebei Province,Hebei Agricultural University,Baoding 071000,China) |
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| Abstract: | In order to explore whether the ABC transporter mediates the tolerance mechanism of Wickerhamomyces anomalus isolated from Populus tomentosa phyllosphere to BDE-3 exposure,the Wapdr15 gene was cloned,sequenced and analyzed on its coding protein structure.The results showed that the length of the coding sequence of Wapdr15 gene was 4536bp,encoding a full molecular transmembrane protein with the structure of TMD1-NBD1-TMD2-NBD2.Tobacco tissue culture seedlings with Wapdr15 gene were obtained via agrobacterium-mediated transformation,and the BDE-3 exposure stress test showed a significant increase in tolerance to BDE-3 compared to the control,revealing that the Wapdr15 might be involved in the process of detoxification of W.anomalus to BDE-3. |
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| Keywords: | Populus tomentosa phyllosphere Wickerhamomyces anomalus ABC transporter BDE-3 |
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